PMID- 11553118
OWN - NLM
STAT- MEDLINE
DCOM- 20030130
LR  - 20191210
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 45
IP  - 2
DP  - 2001 Aug
TI  - Human potency predictions for aldehydes using the local lymph node assay.
PG  - 89-94
AB  - The murine local lymph node assay (LLNA) assesses skin sensitization potential as 
      a function of proliferative responses induced in lymph nodes draining the site of 
      topical exposure to test chemical. It has been shown that interpolation of LLNA 
      dose-response data to define the concentration of test chemical required to 
      induce a 3-fold stimulation of proliferation (EC3) offers the prospect of a 
      quantitative index of the relative potency of a contact allergen. Initial studies 
      have demonstrated that there exists a strong (inverse) correlation between EC3 
      values and contact allergenic potency in humans. Thus, materials with a low EC3 
      value were more potent contact allergens in humans. However, it is necessary to 
      examine a wide range of allergens to demonstrate that such correlations are 
      generally true. Thus, in the present study, 10 aldehydes of varying degrees of 
      allergenicity in man were evaluated in the LLNA and their EC3 values derived. 
      Formaldehyde was regarded as the strongest allergen in man and also had the 
      lowest EC3 value, 0.35% (equivalent to 0.93% formalin). In contrast, the 
      extremely weak allergen vanillin and the non-sensitizer ethyl vanillin both had 
      EC3 values of >50%. For the remaining 7 aldehydes, there was a close similarity 
      between what is judged to be their rank order of allergenicity in humans and EC3 
      values derived from analysis of LLNA data. These results support further the 
      utility of EC3 determinations in the LLNA as a measure of the relative potency of 
      a contact allergen.
FAU - Basketter, D A
AU  - Basketter DA
AD  - SEAC Toxicology Unit, Unilever Research, Colworth House, Sharnbrook, 
      Bedfordshire, MK44 1LQ, UK.
FAU - Wright, Z M
AU  - Wright ZM
FAU - Warbrick, E V
AU  - Warbrick EV
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
FAU - Ryan, C A
AU  - Ryan CA
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - White, I R
AU  - White IR
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Aldehydes)
RN  - 0 (Allergens)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Aldehydes/administration & dosage/*adverse effects/*pharmacology
MH  - Allergens/administration & dosage/*adverse effects/*pharmacology
MH  - Animals
MH  - Dermatitis, Allergic Contact/*diagnosis/etiology
MH  - *Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Formaldehyde/administration & dosage/adverse effects/pharmacology
MH  - Humans
MH  - Lymph Nodes/*drug effects
MH  - Lymphocyte Activation/*drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Predictive Value of Tests
EDAT- 2001/09/13 10:00
MHDA- 2003/01/31 04:00
CRDT- 2001/09/13 10:00
PHST- 2001/09/13 10:00 [pubmed]
PHST- 2003/01/31 04:00 [medline]
PHST- 2001/09/13 10:00 [entrez]
AID - cod450206 [pii]
AID - 10.1034/j.1600-0536.2001.045002089.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2001 Aug;45(2):89-94. doi: 
      10.1034/j.1600-0536.2001.045002089.x.