PMID- 11553298
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 14
IP  - 3
DP  - 2001 Aug
TI  - BDNF increases the early expression of TRH mRNA in fetal TrkB+ hypothalamic 
      neurons in primary culture.
PG  - 483-94
AB  - Known effects of neurotrophins in the developing central nervous system include 
      induction or regulation of peptide expression. Hypothalamic postmitotic 
      thyrotropin-releasing hormone (TRH)-producing neurons may require neurotrophins 
      for survival and/or differentiation. This issue was investigated using primary 
      cell cultures derived from 17-day-old fetal rat hypothalamus seeded in serum-free 
      medium and analysed up to 4 days in vitro culture. Neurotrophin receptor (TrkB 
      and TrkC) mRNA expression was detected by RT-PCR in fetal hypothalamus and 
      throughout the culture period. Western blots confirmed the expression of the 
      full-length proteins in vitro. Semi-quantitative RT-PCR showed that the addition 
      of brain-derived neurotrophic factor (BDNF) increases TRH mRNA levels while the 
      addition of neurotrophin-3 does not. TRH cell content was not modified. Studies 
      on the effect of cell density or homologous conditioned medium demonstrated that 
      endogenous factors probably contribute to determine TRH mRNA levels. One of these 
      factors was BDNF because basal TRH mRNA levels were reduced by the addition of a 
      Trk inhibitor or anti-BDNF. TrkB mRNA was expressed in 27% of cells and TRH mRNA 
      in 2% of cells. The number of TRH+ cells was not affected by BDNF treatment. 
      Forty-eight per cent of TRH neurons contained TrkB mRNA; these neurons had higher 
      amounts of TRH mRNA than TrkB- neurons. Only TrkB+ cells responded to BDNF by 
      increasing their TRH mRNA levels suggesting that BDNF may directly affect TRH 
      biosynthesis. In conclusion, fetal hypothalamic TRH neurons are probably 
      heterogeneous in regard to the neurotrophic factors enhancing peptide and mRNA 
      levels. BDNF enhances TRH mRNA levels in a population of TrkB+ fetal hypothalamic 
      TRHergic neurons in primary culture. However, additional influences may be 
      necessary for the establishment of peptide phenotype in the TrkB+ neurons.
FAU - Guerra-Crespo, M
AU  - Guerra-Crespo M
AD  - Departamento de Genetica y Fisiologia Molecular, Instituto de Biotecnologia, 
      Universidad Nacional Autonoma de Mexico, A.P. 510-3, Cuernavaca, Mor., 62271, 
      Mexico.
FAU - Ubieta, R
AU  - Ubieta R
FAU - Joseph-Bravo, P
AU  - Joseph-Bravo P
FAU - Charli, J L
AU  - Charli JL
FAU - Perez-Martinez, L
AU  - Perez-Martinez L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 5Y5F15120W (Thyrotropin-Releasing Hormone)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Count
MH  - Cells, Cultured
MH  - Culture Media, Conditioned
MH  - Digoxigenin
MH  - Hypothalamus/cytology/drug effects/*metabolism
MH  - Neurons/drug effects/*metabolism
MH  - Precipitin Tests
MH  - RNA, Messenger/*biosynthesis
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics/*metabolism
MH  - Receptor, trkC/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thyrotropin-Releasing Hormone/*biosynthesis
EDAT- 2001/09/13 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/09/13 10:00
PHST- 2001/09/13 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/09/13 10:00 [entrez]
AID - ejn1657 [pii]
AID - 10.1046/j.0953-816x.2001.01657.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2001 Aug;14(3):483-94. doi: 10.1046/j.0953-816x.2001.01657.x.