PMID- 1155373
OWN - NLM
STAT- MEDLINE
DCOM- 19751106
LR  - 20190509
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 64
IP  - 1
DP  - 1975 Jul
TI  - The effects of polyanions on NBT Reductions hexose monophosphate shunt activity, 
      and ultrastructure of polymorphonuclear leukocytes.
PG  - 34-40
AB  - Heparin causes enhanced nitroblue tetrazolium (NBT) reduction by 
      polymorphonuclear leukocytes (PMN's). To determine the mechanism of this 
      stimulation, samples of 1 to 3 x 10(7) PMN's were incubated with various 
      concentrations of heparin, chondroitin sulfate A (CSA), and chondroitin sulfate B 
      (CSB), with and without NBT. The effect of the polyanions (PA) on PMN hexose 
      monophosphate shunt (HMPS) activity was determined by the production of 14CO2 
      from glucose-1-14C by the leukocytes. NBT reduction was evaluated histochemically 
      and spectrophotometrically at 515 mmu. Samples of PMN's in heparin and 
      heparin-NBT mixtures were examined by electron microscopy after various 
      incubation periods. Increased NBT reductions by PMN's was found when leukocytes 
      were incubated with heparin, CSA, and CSB, but these compounds had no effect on 
      the HMPS activity of PMN's unless NBT was added. Electron microscopy of samples 
      that contained heparin-NBT revealed an insoluble complex that was phagocytosed by 
      the leukocytes. The stimulation of PMN oxidative metabolism and NBT reduction 
      that follows incubation with PA-NBT appears to be directly related to ingestion 
      of this particulate complex by the leukocytes.
FAU - Czarnetzki, B M
AU  - Czarnetzki BM
FAU - Cowan, D H
AU  - Cowan DH
FAU - Belcher, R W
AU  - Belcher RW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Hexosephosphates)
RN  - 0 (Pentosephosphates)
RN  - 0 (Tetrazolium Salts)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 298-83-9 (Nitroblue Tetrazolium)
RN  - 9005-49-6 (Heparin)
RN  - 9007-27-6 (Chondroitin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Carbon Dioxide/analysis
MH  - Chondroitin/*pharmacology
MH  - Glucose/*metabolism
MH  - Heparin/*pharmacology
MH  - Hexosephosphates/*metabolism
MH  - Humans
MH  - Leukocytes/drug effects/metabolism/*ultrastructure
MH  - Microscopy, Electron
MH  - Nitroblue Tetrazolium/*metabolism
MH  - Pentosephosphates/*metabolism
MH  - Phagocytosis
MH  - Spectrophotometry
MH  - Tetrazolium Salts/*metabolism
EDAT- 1975/07/01 00:00
MHDA- 1975/07/01 00:01
CRDT- 1975/07/01 00:00
PHST- 1975/07/01 00:00 [pubmed]
PHST- 1975/07/01 00:01 [medline]
PHST- 1975/07/01 00:00 [entrez]
AID - 10.1093/ajcp/64.1.34 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1975 Jul;64(1):34-40. doi: 10.1093/ajcp/64.1.34.