PMID- 11554742
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 287
IP  - 2
DP  - 2001 Sep 21
TI  - In vitro analysis of transcriptional repression of the mouse mammary tumor virus 
      promoter.
PG  - 402-10
AB  - Transcription of the proviral DNA of mouse mammary tumor virus (MMTV) is induced 
      by several classes of hormone-activated steroid receptor proteins. Basal promoter 
      activity in the absence of receptor-mediated activation is selectively repressed 
      by a distal negative regulatory element (dNRE) centered approximately 400 bp 
      upstream of the transcription initiation site. An in vitro transcription system 
      based on synthetic T-free cassette templates was developed to assess MMTV 
      promoter activity, and dNRE-mediated repression was partially reconstituted with 
      this system. Repression was observed with templates in which the dNRE was present 
      in several sequence contexts. The activity of transcription preinitiation 
      complexes formed in vitro in the presence of the dNRE could not be distinguished 
      from that of complexes formed in its absence as assessed by the kinetics of 
      transcript accumulation after addition of nucleoside triphosphates to preformed 
      preinitiation complexes. dNRE-mediated repression in vitro appeared to be the 
      result of decreased efficiency of assembly of functional transcription complexes 
      on the MMTV promoter. However, repression could not be explained by inhibition of 
      assembly of TATA-binding protein or transcription factor IIB into transcription 
      complexes, as neither protein decreased the extent of repression when supplied in 
      excess as a purified recombinant protein.
CI  - Copyright 2001 Academic Press.
FAU - Kang, C J
AU  - Kang CJ
AD  - School of Life Science and Biotechnology, Kyung Hee University, Yongin, Kyunggido 
      449-701, Korea. cjkang@khu.ac.kr
FAU - Peterson, D O
AU  - Peterson DO
LA  - eng
GR  - CA32695/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Transcription Factor TFIIB)
RN  - 0 (Transcription Factor TFIID)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, TFII)
SB  - IM
MH  - *Gene Expression Regulation, Viral
MH  - Gene Silencing
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Promoter Regions, Genetic/*genetics/physiology
MH  - Terminal Repeat Sequences/genetics
MH  - Transcription Factor TFIIB
MH  - Transcription Factor TFIID
MH  - Transcription Factors/metabolism
MH  - Transcription Factors, TFII/metabolism
MH  - Transcription, Genetic/*genetics
EDAT- 2001/09/14 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/09/14 10:00
PHST- 2001/09/14 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/09/14 10:00 [entrez]
AID - S0006-291X(01)95601-3 [pii]
AID - 10.1006/bbrc.2001.5601 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Sep 21;287(2):402-10. doi: 
      10.1006/bbrc.2001.5601.