PMID- 11554990
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190921
IS  - 0105-6263 (Print)
IS  - 0105-6263 (Linking)
VI  - 24
IP  - 5
DP  - 2001 Oct
TI  - Predictive value of chromatin decondensation in vitro on fertilization rate after 
      intracytoplasmic sperm injection (ICSI).
PG  - 311-6
AB  - This study was undertaken to identify the relationship between sperm chromatin 
      decondensation after incubation with sodium dodecyl sulphate (SDS) and ethylene 
      diamine tetra acetic acid (EDTA), or heparin at various points of time. Likewise, 
      this study will determine chromatin stability within definite time intervals, 
      chromatin decondensation after intracytoplasmic sperm injection (ICSI), and 
      whether chromatin decondensation in vitro could be used as a predictive test for 
      fertilization capability after ICSI. Sixty-five infertile couples undergoing ICSI 
      therapy were included in this prospective study. Male factor infertility was the 
      main indication for inclusion. One millilitre from each semen sample after 
      washing was mixed with SDS-EDTA (group 1) or SDS/heparin (group 2) and incubated 
      for 120 min. Many smears were made within 10 min of mixing the spermatozoa with 
      detergent and the reducing agents and at the following points of time 30, 60 and 
      120 min and after 24 h. Chromatin decondensation was evaluated after staining 
      with acridine orange (AO). The mean percentage of uncondensed chromatin of 
      spermatozoa in the semen sample in the first group before addition of SDS/EDTA 
      was 26.1 +/- 19.0 and 22.3 +/- 18.9% in the second one. After incubation of 
      spermatozoa for 30, 60 and 120 min and 24 h, the chromatin decondensation 
      increased in the first group to 64.0 +/- 28.6, 83.0 +/- 21.1, 87.9 +/- 14.6, 92.1 
      +/- 16.2 and 98.0 +/- 6.75%, respectively. The corresponding values in the second 
      group were 69.5 +/- 29.9, 78.6 +/- 22.4, 86.9 +/- 17.1, 95.13 +/- 6.5 and 98.3 
      +/- 5.6%. On the other hand, no correlations were found between the chromatin 
      decondensation or chromatin decondensation rate in vitro and the fertilization 
      rates in all investigated groups. In conclusion, neither the chromatin 
      decondensation ability in vitro nor the rate of chromatin decondensation between 
      various points of time after using SDS/EDTA, SDS/heparin could predict the 
      chromatin decondensation of spermatozoa (fertilization capability) after ICSI.
FAU - Hammadeh, M E
AU  - Hammadeh ME
AD  - Department of Obstetrics and Gynaecology, Medical College, University of 
      Saarland, 66421 Homburg/Saar, Germany. frmham@med-rz.uni-sb.de
FAU - Al-Hasani, S
AU  - Al-Hasani S
FAU - Gauss, C
AU  - Gauss C
FAU - Rosenbaum, P
AU  - Rosenbaum P
FAU - Georg, T
AU  - Georg T
FAU - Diedrich, K
AU  - Diedrich K
FAU - Schmidt, W
AU  - Schmidt W
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - England
TA  - Int J Androl
JT  - International journal of andrology
JID - 8000141
RN  - 0 (Chromatin)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 9005-49-6 (Heparin)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Adult
MH  - Chromatin/*physiology
MH  - Edetic Acid/pharmacology
MH  - Female
MH  - Fertility
MH  - *Fertilization
MH  - Heparin/pharmacology
MH  - Humans
MH  - Male
MH  - Predictive Value of Tests
MH  - Pregnancy
MH  - Prospective Studies
MH  - Sodium Dodecyl Sulfate/pharmacology
MH  - *Sperm Injections, Intracytoplasmic
MH  - Spermatozoa/drug effects/*physiology
EDAT- 2001/09/14 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/14 10:00
PHST- 2001/09/14 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/14 10:00 [entrez]
AID - ija308 [pii]
AID - 10.1046/j.1365-2605.2001.00308.x [doi]
PST - ppublish
SO  - Int J Androl. 2001 Oct;24(5):311-6. doi: 10.1046/j.1365-2605.2001.00308.x.