PMID- 11555401
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 54
IP  - 4
DP  - 2001 Oct
TI  - Immunostimulatory DNA from Paracoccidioides brasiliensis acts as T-helper 1 
      promoter in susceptible mice.
PG  - 348-56
AB  - Th1 immune responses afford protection against some pathogens like the fungus P. 
      brasiliensis (P.b.), etiological agent of Paracoccidioidomycosis (PCM). It is 
      well known that nonmethylated CpG sequences from bacterial DNA have 
      immunomodulatory properties and can be used as a Th1-promoting adjuvant. By 
      analyzing the available gene sequences of P.b. we observed a high number of 
      unmethylated CpG dinucleotides. In a murine model of the PCM infection, the 
      isogenic mouse strain known to be susceptible presents a predominant Th2 pattern. 
      In order to access the possibility of the genomic DNA to act as a Th1-promoting 
      adjuvant, in vitro assays were made and indicated a significant increase in 
      phagocytosis when the macrophages were stimulated with DNA from P.b. and in vivo 
      assays of a decreased production of antibodies antigp43, the main antigen of the 
      PCM system. The analysis of the antibody isotypes and the cytokine production 
      suggested a Th1 modulation in the susceptible animals. Thus, when mice were 
      infected with fungus plus synthetic oligodeoxynucleotide (ODN), made from the 
      available sequence of gp43, a decrease in the fungus dissemination was observed. 
      Results herein described suggest that genomic DNA from P.b. could have a 
      immunostimulatory function as a Th-1-promoting adjuvant in susceptible mice.
FAU - Souza, M C
AU  - Souza MC
AD  - Department of Microbiology, Immunology and Parasitology-Federal University of Sao 
      Paulo (UNIFESP), Sao Paulo, SP, Brazil.
FAU - Correa, M
AU  - Correa M
FAU - Almeida, S R
AU  - Almeida SR
FAU - Lopes, J D
AU  - Lopes JD
FAU - Camargo, Z P
AU  - Camargo ZP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Fungal Vaccines)
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (Vaccines, DNA)
RN  - 207137-56-2 (Interleukin-4)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - Antibodies, Fungal/blood
MH  - *Antigens, Fungal
MH  - Cells, Cultured
MH  - CpG Islands/*immunology
MH  - DNA Methylation
MH  - DNA, Fungal/*immunology
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - *Fungal Proteins
MH  - Fungal Vaccines/*immunology
MH  - Glycoproteins/*genetics
MH  - Interferon-gamma/biosynthesis/immunology/pharmacology
MH  - Interleukin-4/biosynthesis
MH  - Macrophages, Peritoneal/cytology/immunology/microbiology
MH  - Male
MH  - Mice
MH  - Nitric Oxide/biosynthesis
MH  - Oligosaccharides/*genetics
MH  - Paracoccidioides/genetics/growth & development/immunology
MH  - Paracoccidioidomycosis/*immunology/prevention & control
MH  - Phagocytosis/immunology
MH  - Th1 Cells/*immunology
MH  - Vaccines, DNA/*immunology
EDAT- 2001/09/14 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/09/14 10:00
PHST- 2001/09/14 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/09/14 10:00 [entrez]
AID - sji937 [pii]
AID - 10.1046/j.1365-3083.2001.00937.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2001 Oct;54(4):348-56. doi: 10.1046/j.1365-3083.2001.00937.x.