PMID- 11556399
OWN - NLM
STAT- MEDLINE
DCOM- 20020222
LR  - 20191025
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 23
IP  - 1
DP  - 2001
TI  - Sequence and genetic analyses of the 3' terminus and integration sites of the 
      RIII/Sa mouse mammary tumor (MMTV) exogenous provirus.
PG  - 35-43
AB  - The RIII/Sa mouse mammary tumor virus (MMTV) is one of several exogenous MMTV 
      viruses transmitted to suckling mice through the milk. We report herein the 
      nucleotide sequence of the exogenous RIII/Sa provirus from the central Eco RI 
      site through to the end of the U5 region encoded by the 3' LTR. We also provide a 
      detailed sequence analysis often different 3' terminal exogenous MMTV proviral 
      integration sites within mammary tumor DNA obtained by the technique of genome 
      walking. Using a combination of Southern blotting with 3' end probes and PCR 
      utilizing a unique RIII/Sa specific 3' primer, we confirm that the RIII/Sa 
      provirus integrates multiple times in mouse mammary tumors but that little or no 
      integration occurs in various normal tissues. Southern blotting analyses with 3' 
      end probes also indicate that RIII/Sa mice contain two additional endogenous MMTV 
      loci, mtv-6 and mtv- 17, not previously reported. The combined genetic analyses 
      reported herein distinguish between exogenous proviral integrants and endogenous 
      germline MMTV.
FAU - Popken-Harris, P
AU  - Popken-Harris P
AD  - David F. Hickok Memorial Cancer Research Laboratory, Abbott Northwestern 
      Hospital, the Minneapolis, MN 55407, USA.
FAU - Pliml, L
AU  - Pliml L
FAU - Harris, L
AU  - Harris L
LA  - eng
SI  - GENBANK/AF071010
SI  - GENBANK/AF263910
SI  - GENBANK/AF263911
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Southern/methods
MH  - Cloning, Molecular
MH  - *DNA, Viral
MH  - Female
MH  - Gene Library
MH  - Genes, Viral
MH  - Mammary Tumor Virus, Mouse/*genetics/isolation & purification
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proviruses/*genetics
MH  - Sequence Analysis, DNA
MH  - *Terminal Repeat Sequences
MH  - *Virus Integration
EDAT- 2001/09/15 10:00
MHDA- 2002/02/23 10:01
CRDT- 2001/09/15 10:00
PHST- 2001/09/15 10:00 [pubmed]
PHST- 2002/02/23 10:01 [medline]
PHST- 2001/09/15 10:00 [entrez]
AID - 10.1023/a:1011175112113 [doi]
PST - ppublish
SO  - Virus Genes. 2001;23(1):35-43. doi: 10.1023/a:1011175112113.