PMID- 11556864
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20190826
IS  - 0886-4470 (Print)
IS  - 0886-4470 (Linking)
VI  - 127
IP  - 9
DP  - 2001 Sep
TI  - Immunohistological expression of interleukin 16 in human tonsils.
PG  - 1120-5
AB  - BACKGROUND: Interleukin 16 (IL-16) acts highly chemotactic on CD4-bearing cells. 
      Besides chemotaxis, IL-16 has numerous immunomodulatory effects, and not only on 
      T cells. OBJECTIVE: To determine IL-16 expression in human tonsils. METHODS: 
      Tonsillar follicles were immunohistologically characterized to elicit a possible 
      cellular source of IL-16 expression. RESULTS: The mantle zone of immature and 
      mature B cells was CD22 immunoreactive (ir), whereas the germinal center of 
      activated B cells was CD23-ir. Plasma cells that were CD38-ir were observed 
      extrafollicularly beneath the epithelium and within the germinal center. T cells 
      were found most frequently in the extrafollicular space, with a majority of CD4 
      cells. CD68-ir macrophages were predominantly found within the germinal center. 
      Immunostaining of anti-IL-16 revealed strong cytoplasmatic reactivity of 
      extrafollicular cells and of cells at the outer rim of the mantle zone. Numerous 
      cells adherent to the stratified squamous epithelium were IL-16-ir as well. 
      Double immunostaining identified CD4(+) T cells as the major cellular source of 
      IL-16 expression. Furthermore, a population of CD22(+) B cells at the outer rim 
      of the mantle zone expressed IL-16 as well. CONCLUSIONS: Interleukin 16 was 
      mainly expressed in a typical CD4-like pattern in human tonsils. Our data 
      strongly suggest that CD4(+) lymphocytes constitute the major cellular source for 
      IL-16. We hypothesize that the double-immunostained CD4-ir and IL-16-ir cells 
      represent activated T cells. Because CD22(+) B cells at the outer rim of the 
      mantle zone expressed IL-16 as well, we conclude that this area might constitute 
      the locus of IL-16-mediated B-cell differentiation.
FAU - Kramer, M F
AU  - Kramer MF
AD  - Department of Oto-Rhino-Laryngology/Head and Neck Surgery, 
      Ludwig-Maximilians-University, Klinikum Grosshadern, Marchioninistr.15, 81377 
      Munich, Germany. mkramer@hno.med.uni-muenchen.de
FAU - Mack, B
AU  - Mack B
FAU - Rasp, G
AU  - Rasp G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
RN  - 0 (Interleukin-16)
SB  - IM
MH  - Child
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-16/analysis/*biosynthesis
MH  - Palatine Tonsil/chemistry/*immunology/pathology
EDAT- 2001/09/15 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/09/15 10:00
PHST- 2001/09/15 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/09/15 10:00 [entrez]
AID - ooa00240 [pii]
AID - 10.1001/archotol.127.9.1120 [doi]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 2001 Sep;127(9):1120-5. doi: 
      10.1001/archotol.127.9.1120.