PMID- 11559002
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20061115
IS  - 1046-2023 (Print)
IS  - 1046-2023 (Linking)
VI  - 25
IP  - 1
DP  - 2001 Sep
TI  - Fluorescence in situ hybridization analysis of transcript dynamics in cells.
PG  - 111-8
AB  - Since the first description of in situ hybridization in 1969 the technique has 
      advanced to allow sensitive detection of DNA and mRNA molecules at the cellular 
      and subcellular levels. In particular fluorescence in situ hybridization (FISH) 
      has become a frequently used tool in basic and applied biomedical research since 
      detection is sensitive and allows discrimination of multiple targets in the same 
      sample. By using RNA-FISH we have been able to detect primary transcripts of the 
      human embryonic, fetal, and adult globins in erythroid cells to study the 
      competitive transcription mechanism or variegated expression patterns of the 
      human beta-globin locus. We have correlated such expression patterns with other 
      parameters such as cell type, cell cycle, replication, and stage of 
      differentiation by simultaneous detection of, e.g., incorporated BrdUTPs, 
      proteins (e.g., cyclins A and E, PCNA, histones), and globin (primary) 
      transcripts and/or locus integration sites. Thus a combination of FISH and 
      immunofluorescence methods allow the visualization of different processes taking 
      place in the nucleus relative to each other in terms of three-dimensional space 
      and structure and time (development, cell cycle).
CI  - Copyright 2001 Academic Press.
FAU - van de Corput, M P
AU  - van de Corput MP
AD  - Department of Cell Biology and Genetics, Faculty of Medicine and Health Sciences, 
      Erasmus University, Dr. Molewaterplein 50, Rotterdam, 3015 GE, The Netherlands.
FAU - Grosveld, F G
AU  - Grosveld FG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods
JT  - Methods (San Diego, Calif.)
JID - 9426302
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 9004-22-2 (Globins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*isolation & purification/metabolism
MH  - Globins/biosynthesis
MH  - *In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence/*methods
MH  - RNA Precursors/*isolation & purification/metabolism
MH  - RNA, Messenger/*isolation & purification/metabolism
MH  - Transcription, Genetic
EDAT- 2001/09/18 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/18 10:00
PHST- 2001/09/18 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/18 10:00 [entrez]
AID - S1046-2023(01)91220-4 [pii]
AID - 10.1006/meth.2001.1220 [doi]
PST - ppublish
SO  - Methods. 2001 Sep;25(1):111-8. doi: 10.1006/meth.2001.1220.