PMID- 11559020
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20131121
IS  - 0041-008X (Print)
IS  - 0041-008X (Linking)
VI  - 175
IP  - 3
DP  - 2001 Sep 15
TI  - Effects of phospholipid surfactant on apoptosis induction by respirable quartz 
      and kaolin in NR8383 rat pulmonary macrophages.
PG  - 217-25
AB  - Apoptosis was measured in rat alveolar macrophage NR8383 cells challenged in 
      vitro with respirable quartz or kaolin dust and with the dusts pretreated with 
      dipalmitoyl phosphatidylcholine (DPPC) to model conditioning of respired dusts by 
      interaction with a primary phospholipid component of pulmonary surfactant. Quartz 
      dust is known to induce apoptosis in vitro and in vivo. For this study, quartz 
      and kaolin were compared as dusts of similar cytotoxicity in some in vitro assays 
      but of differing pathogenic potential: quartz can cause significant pulmonary 
      fibrosis while kaolin generally does not. NR8383 cells exposed to native quartz 
      at concentrations from 50 to 400 microg/ml for 6 h showed a dose-dependent 
      increase in apoptosis measured by the TdT-mediated dUTP-fluorescein nick end 
      labeling (TUNEL), cell death ELISA, and DNA ladder formation assays, while native 
      kaolin induced significant response only at the higher concentrations and only in 
      the TUNEL and ELISA assays. For cell challenge from 6 h to 5 days at 100 
      microg/ml of dust, quartz was active at all times while kaolin was active only at 
      5 days. DPPC pre-treatment suppressed quartz activity until 3 days and kaolin 
      activity through 5 days. Cellular release of lactate dehydrogenase, measured in 
      parallel experiments to compare dust apoptotic and necrotic activities, indicated 
      that components of serum as well as surfactant may affect kaolin in vitro 
      expression of those activities.
FAU - Gao, N
AU  - Gao N
AD  - National Institute for Occupational Safety and Health, 1095 Willowdale Road, 
      Morgantown, WV 26505, USA.
FAU - Keane, M J
AU  - Keane MJ
FAU - Ong, T
AU  - Ong T
FAU - Ye, J
AU  - Ye J
FAU - Miller, W E
AU  - Miller WE
FAU - Wallace, W E
AU  - Wallace WE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Drug Combinations)
RN  - 0 (Dust)
RN  - 14808-60-7 (Quartz)
RN  - 24H4NWX5CO (Kaolin)
RN  - 2644-64-6 (1,2-Dipalmitoylphosphatidylcholine)
RN  - 9007-49-2 (DNA)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - 1,2-Dipalmitoylphosphatidylcholine/*pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line
MH  - DNA/analysis
MH  - DNA Fragmentation
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Drug Interactions
MH  - Dust
MH  - Enzyme-Linked Immunosorbent Assay
MH  - In Situ Nick-End Labeling
MH  - Kaolin/*toxicity
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Macrophages, Alveolar/*drug effects/enzymology/pathology
MH  - Male
MH  - Quartz/*toxicity
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2001/09/18 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/09/18 10:00
PHST- 2001/09/18 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/09/18 10:00 [entrez]
AID - S0041-008X(01)99249-X [pii]
AID - 10.1006/taap.2001.9249 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2001 Sep 15;175(3):217-25. doi: 10.1006/taap.2001.9249.