PMID- 11559442
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20071115
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 8
DP  - 2001 Aug
TI  - Interleukin-12 upregulates the IL-18Rbeta chain in BALB/c thymocytes.
PG  - 635-42
AB  - Interleukin-18 (IL-18) is a proinflammatory cytokine that plays an important role 
      in innate immunity and specific Th1 immune responses. During infection, IL-18 is 
      produced by activated macrophages and primarily induces interferon-gamma 
      (IFN-gamma) production in synergy with IL-12 in primed T cells and natural killer 
      (NK) cells. IL-18 exerts its function by binding to a specific receptor complex 
      (IL-18R) consisting of two subunits, the IL-18Ralpha chain and the IL-18Rbeta 
      chain. Two individual mechanisms for synergism between IL-12 and IL-18 have been 
      reported. First, IL-12-induced expression of the IL-18 receptor complex was 
      described. Second, IL-18 and IL-12 together induced expression of target genes on 
      a transcriptional level. In this study, we show that freshly isolated thymocytes 
      from BALB/c mice constitutively expressed mRNA for the IL-18Ralpha chain but not 
      for the IL-18Rbeta chain. These thymocytes were unresponsive to IL-18. Treatment 
      with a combination of concanavalinA (ConA) plus IL-12 did not affect expression 
      of the IL-18Ralpha chain but strongly induced expression for IL-18Rbeta mRNA, 
      rendering these thymocytes highly responsive to IL-18 treatment. Thus, the 
      synergistic effects of IL-12 and IL-18 on BALB/c mouse thymocytes resulted from 
      the induction and regulation of the IL-18Rbeta chain and not the IL-18Ralpha 
      chain.
FAU - Neumann, D
AU  - Neumann D
AD  - Pharmacology OE 5320, Hannover Medical School, D-30623 Hannover, Germany.
FAU - Martin, M U
AU  - Martin MU
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Il18r1 protein, mouse)
RN  - 0 (Immunologic Factors)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Female
MH  - Immunologic Factors/physiology
MH  - Interleukin-12/*physiology
MH  - Interleukin-18/genetics/*metabolism
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Interleukin/*biosynthesis/genetics
MH  - Receptors, Interleukin-18
MH  - T-Lymphocytes/*immunology/*metabolism
MH  - Up-Regulation/genetics/*immunology
EDAT- 2001/09/18 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/18 10:00
PHST- 2001/09/18 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/18 10:00 [entrez]
AID - 10.1089/10799900152547902 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Aug;21(8):635-42. doi: 10.1089/10799900152547902.