PMID- 11560993
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20240213
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 194
IP  - 6
DP  - 2001 Sep 17
TI  - Dendritic cells induce peripheral T cell unresponsiveness under steady state 
      conditions in vivo.
PG  - 769-79
AB  - Dendritic cells (DCs) have the capacity to initiate immune responses, but it has 
      been postulated that they may also be involved in inducing peripheral tolerance. 
      To examine the function of DCs in the steady state we devised an antigen delivery 
      system targeting these specialized antigen presenting cells in vivo using a 
      monoclonal antibody to a DC-restricted endocytic receptor, DEC-205. Our 
      experiments show that this route of antigen delivery to DCs is several orders of 
      magnitude more efficient than free peptide in complete Freund's adjuvant (CFA) in 
      inducing T cell activation and cell division. However, T cells activated by 
      antigen delivered to DCs are not polarized to produce T helper type 1 cytokine 
      interferon gamma and the activation response is not sustained. Within 7 d the 
      number of antigen-specific T cells is severely reduced, and the residual T cells 
      become unresponsive to systemic challenge with antigen in CFA. Coinjection of the 
      DC-targeted antigen and anti-CD40 agonistic antibody changes the outcome from 
      tolerance to prolonged T cell activation and immunity. We conclude that in the 
      absence of additional stimuli DCs induce transient antigen-specific T cell 
      activation followed by T cell deletion and unresponsiveness.
FAU - Hawiger, D
AU  - Hawiger D
AD  - Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 
      10021, USA.
FAU - Inaba, K
AU  - Inaba K
FAU - Dorsett, Y
AU  - Dorsett Y
FAU - Guo, M
AU  - Guo M
FAU - Mahnke, K
AU  - Mahnke K
FAU - Rivera, M
AU  - Rivera M
FAU - Ravetch, J V
AU  - Ravetch JV
FAU - Steinman, R M
AU  - Steinman RM
FAU - Nussenzweig, M C
AU  - Nussenzweig MC
LA  - eng
GR  - R01 AI013013/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
RN  - EC 3.2.1.- (hen egg lysozyme)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD/immunology
MH  - B7-2 Antigen
MH  - CD40 Antigens/immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - *Lectins, C-Type
MH  - Lymphocyte Activation/*immunology
MH  - Lymphocytes/immunology
MH  - Membrane Glycoproteins/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Minor Histocompatibility Antigens
MH  - Muramidase/immunology
MH  - Receptors, Cell Surface/immunology
MH  - Spleen/cytology
MH  - T-Lymphocytes/*immunology
PMC - PMC2195961
EDAT- 2001/09/19 10:00
MHDA- 2001/10/26 10:01
PMCR- 2002/03/17
CRDT- 2001/09/19 10:00
PHST- 2001/09/19 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/09/19 10:00 [entrez]
PHST- 2002/03/17 00:00 [pmc-release]
AID - 011111 [pii]
AID - 10.1084/jem.194.6.769 [doi]
PST - ppublish
SO  - J Exp Med. 2001 Sep 17;194(6):769-79. doi: 10.1084/jem.194.6.769.