PMID- 11561017 OWN - NLM STAT- MEDLINE DCOM- 20011004 LR - 20170214 IS - 0022-1554 (Print) IS - 0022-1554 (Linking) VI - 49 IP - 10 DP - 2001 Oct TI - Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies. PG - 1321-2 AB - Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants. FAU - Dundas, S R AU - Dundas SR AD - Department of Clinical and Surgical Sciences, University of Edinburgh, Scotland, UK. srdundas@yahoo.co.uk FAU - Boyle, S AU - Boyle S FAU - Bellamy, C O AU - Bellamy CO FAU - Hawkins, W AU - Hawkins W FAU - Garden, O J AU - Garden OJ FAU - Ross, J A AU - Ross JA FAU - Bickmore, W AU - Bickmore W LA - eng PT - Journal Article PL - United States TA - J Histochem Cytochem JT - The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society JID - 9815334 SB - IM MH - Chromosome Painting MH - Female MH - Fluorescent Antibody Technique, Indirect MH - Humans MH - Liver/*ultrastructure MH - *Liver Transplantation MH - Male MH - Phenotype MH - *Y Chromosome EDAT- 2001/09/19 10:00 MHDA- 2001/10/05 10:01 CRDT- 2001/09/19 10:00 PHST- 2001/09/19 10:00 [pubmed] PHST- 2001/10/05 10:01 [medline] PHST- 2001/09/19 10:00 [entrez] AID - 10.1177/002215540104901015 [doi] PST - ppublish SO - J Histochem Cytochem. 2001 Oct;49(10):1321-2. doi: 10.1177/002215540104901015.