PMID- 11561150
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20171101
IS  - 1018-1172 (Print)
IS  - 1018-1172 (Linking)
VI  - 38
IP  - 5
DP  - 2001 Sep-Oct
TI  - Smooth muscle cells influence monocyte response to LDL as well as their adhesion 
      and transmigration in a coculture model of the arterial wall.
PG  - 479-91
AB  - We investigated the possible interference of smooth muscle cells with monocyte 
      response to LDL as well as with their adhesion and transmigration in a coculture 
      of porcine endothelial and smooth muscle cells. Lysophosphatidylcholine (LPC), a 
      component of oxidized LDL (oxLDL), stimulated the adhesion of THP-1 cells to 
      endothelial cells both in mono- and in coculture with smooth muscle cells. When 
      THP-1 cells were incubated with endothelial cells in the presence of copper 
      oxLDL, their adhesion was increased, but only in coculture. The addition of 
      sodium nitroprusside (SNP) together with oxLDL markedly increased the adhesion of 
      THP-1 cells in coculture. Close proximity between endothelial and smooth muscle 
      cells was necessary to observe that effect. Furthermore, this increase in 
      adhesion of THP-1 cells can, at least in part, be attributed to the augmented 
      production of monocyte chemoattractant protein-1 (MCP-1) observed in coculture 
      under the influence of oxLDL and SNP. The passage of THP-1 cells through the 
      coculture was stimulated by MCP-1 and LPC. These results show that physical 
      contacts or close proximity between endothelial and smooth muscle cells play a 
      key role in the adhesion of monocytes and their infiltration into the intima in 
      response to oxLDL.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Kinard, F
AU  - Kinard F
AD  - Laboratoire de Biologie Cellulaire, Universite Catholique de Louvain, 
      Louvain-La-Neuve, Belgium. Kinard@bani.ucl.ac.be
FAU - Jaworski, K
AU  - Jaworski K
FAU - Sergent-Engelen, T
AU  - Sergent-Engelen T
FAU - Goldstein, D
AU  - Goldstein D
FAU - Van Veldhoven, P P
AU  - Van Veldhoven PP
FAU - Holvoet, P
AU  - Holvoet P
FAU - Trouet, A
AU  - Trouet A
FAU - Schneider, Y J
AU  - Schneider YJ
FAU - Remacle, C
AU  - Remacle C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - J Vasc Res
JT  - Journal of vascular research
JID - 9206092
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Nitric Oxide Donors)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (oxidized low density lipoprotein)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/*drug effects
MH  - Chemokine CCL2/analysis
MH  - Coculture Techniques
MH  - Culture Media
MH  - Endothelium, Vascular/cytology/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Lipoproteins, LDL/*pharmacology
MH  - Lysophosphatidylcholines/pharmacology
MH  - Microscopy, Confocal
MH  - Monocytes/*drug effects/*physiology
MH  - Muscle, Smooth, Vascular/*cytology/*physiology
MH  - Nitric Oxide/pharmacology
MH  - Nitric Oxide Donors/pharmacology
MH  - Pulmonary Artery
MH  - Swine
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2001/09/19 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/09/19 10:00
PHST- 2001/09/19 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/09/19 10:00 [entrez]
AID - 51081 [pii]
AID - 10.1159/000051081 [doi]
PST - ppublish
SO  - J Vasc Res. 2001 Sep-Oct;38(5):479-91. doi: 10.1159/000051081.