PMID- 11561289
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20171213
IS  - 0749-503X (Print)
IS  - 0749-503X (Linking)
VI  - 18
IP  - 13
DP  - 2001 Sep 30
TI  - Functional isolation of the Candida albicans FCR3 gene encoding a bZip 
      transcription factor homologous to Saccharomyces cerevisiae Yap3p.
PG  - 1217-25
AB  - We have isolated a C. albicans gene, named FCR3 (for fluconazole resistance 3), 
      based upon its ability to suppress the FCZ hypersusceptibility of a Saccharomyces 
      cerevisiae mutant strain (JY312) lacking the transcription factors Pdr1p and 
      Pdr3p. The FCR3 ORF (1200 bp) encodes a 399 amino acid protein containing a basic 
      leucine zipper (bZip) domain. Fcr3p displays the highest level of sequence 
      homology with the S. cerevisiae Yap3p protein (34% identity, 45% similarity). We 
      had previously shown that deletion of the PDR5 gene encoding a multidrug 
      transporter completely abolished the ability of FCR3 to suppress the FCZ 
      hypersusceptibility of JY312, suggesting that FCR3 confers FCZ resistance by 
      activating PDR5 expression. We show here that the beta-galactosidase activity of 
      a PDR5 promoter-lacZ construct in JY312 is increased two-fold upon FCR3 
      overexpression, demonstrating that FCR3 regulates PDR5 at the transcriptional 
      level. We also show that FCR3 overexpression not only suppresses the 
      hypersusceptibility of JY312 to 4-nitroquinoline-N-oxide (4-NQO) but also confers 
      higher levels of resistance to this compound as compared to the wild-type KY320 
      strain. Since PDR5 is not involved in 4-NQO resistance, this result indicates 
      that FCR3 can also activate the transcription of other genes that can confer 
      4-NQO resistance. Finally, Northern blot analysis indicates that FCR3 encodes a 
      single 2.4 kb RNA transcript in C. albicans, suggesting that the FCR3 mRNA 
      contains long 5' and/or 3' untranslated regions. The nucleotide sequence of the 
      FCR3 gene has been deposited at GenBank under Accession No. AF342983.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Yang, X
AU  - Yang X
AD  - Institut de Recherches Cliniques de Montreal, Montreal, Quebec, Canada H2W 1R7.
FAU - Talibi, D
AU  - Talibi D
FAU - Weber, S
AU  - Weber S
FAU - Poisson, G
AU  - Poisson G
FAU - Raymond, M
AU  - Raymond M
LA  - eng
SI  - GENBANK/AF342983
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Yeast
JT  - Yeast (Chichester, England)
JID - 8607637
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antifungal Agents)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (G-Box Binding Factors)
RN  - 0 (Membrane Proteins)
RN  - 0 (PDR5 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 8VZV102JFY (Fluconazole)
RN  - EC 3.4.23 (aspartic proteinase A)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.23.25 (YPS1 protein, S cerevisiae)
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - Amino Acid Sequence
MH  - Antifungal Agents/*pharmacology
MH  - Aspartic Acid Endopeptidases/*genetics
MH  - Basic-Leucine Zipper Transcription Factors
MH  - Candida albicans/drug effects/*genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/chemistry/*genetics/*metabolism
MH  - Drug Resistance, Fungal
MH  - Fluconazole/*pharmacology
MH  - G-Box Binding Factors
MH  - Membrane Proteins/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Saccharomyces cerevisiae/drug effects/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Analysis, DNA
MH  - Transcription Factors/chemistry/*genetics/*metabolism
MH  - Transcriptional Activation
EDAT- 2001/09/19 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/19 10:00
PHST- 2001/09/19 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/19 10:00 [entrez]
AID - 10.1002/yea.770 [pii]
AID - 10.1002/yea.770 [doi]
PST - ppublish
SO  - Yeast. 2001 Sep 30;18(13):1217-25. doi: 10.1002/yea.770.