PMID- 11561711
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190605
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 65
IP  - 3
DP  - 2001 Sep
TI  - Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in 
      sera of patients with paracoccidioidomycosis.
PG  - 237-41
AB  - Paracoccidioidomycosis (PCM) is the most frequent systemic mycosis in South 
      America. The disease is characterized by a polyclonal activation of B cells, 
      resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in 
      deep mycosis has been related to the severity of the disease. However, the 
      detection of specific IgE in sera of patients is difficult because of the 
      competition with the IgG. We compared a capture and an indirect enzyme-linked 
      immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis 
      IgE. We found that the capture ELISA presented higher performance and lower 
      background values than the indirect assay, resulting in a significant 
      quantitative discrimination between sera from patients with the 2 major clinical 
      forms of PCM. Patients with the juvenile form presented significantly higher 
      levels of P. brasiliensis IgE, as compared with patients with the adult form. The 
      capture ELISA was used in the follow-up of patients receiving treatment, showing 
      that the levels of specific IgE decreased as the patient's clinical conditions 
      improved.
FAU - Mamoni, R L
AU  - Mamoni RL
AD  - Department of Clinical Pathology, Faculty of Medical Sciences, State University 
      of Campinas, SP, Brasil.
FAU - Rossi, C L
AU  - Rossi CL
FAU - Camargo, Z P
AU  - Camargo ZP
FAU - Blotta, M H
AU  - Blotta MH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Antigens, Fungal/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - *Fungal Proteins
MH  - Glycoproteins/immunology
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Oligosaccharides/immunology
MH  - Paracoccidioides/*immunology/isolation & purification
MH  - Paracoccidioidomycosis/*immunology/microbiology
MH  - Statistics, Nonparametric
EDAT- 2001/09/20 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/09/20 10:00
PHST- 2001/09/20 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/09/20 10:00 [entrez]
AID - 10.4269/ajtmh.2001.65.237 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2001 Sep;65(3):237-41. doi: 10.4269/ajtmh.2001.65.237.