PMID- 11561752
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190921
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 439
IP  - 2
DP  - 2001 Aug
TI  - Expression of MAGE tumour-associated antigens is inversely correlated with tumour 
      differentiation in invasive ductal breast cancers: an immunohistochemical study.
PG  - 127-31
AB  - MAGE (Melanoma antigen E) family gene products encompass tumour-associated 
      antigens (TAAs) recognised by human leukocyte antigen (HLA)-restricted specific 
      T-cells. Agents inducing DNA demethylation, an event typically detectable in 
      cellular de-differentiation processes, were shown to induce the expression of 
      MAGE genes. By using a monoclonal antibody specific for MAGE family gene 
      products, we have studied the expression of these TAAs in a group of 144 patients 
      with invasive ductal breast cancers. Immunohistochemical data were correlated 
      with tumour differentiation, lymphatic vessel invasion, oestrogen receptor 
      expression, intratumoural necrosis, lymphocytic infiltration, perineural 
      invasion, tumour microcalcifications and axillary lymph node metastases. MAGE 
      immunoreactivity was undetectable in non-neoplastic cells. In poorly 
      differentiated cancers positive staining was observed in 30/63 cases (47.6%) as 
      compared with 13/51 (25.4%) and 5/30 (16.6%) in moderately and 
      well-differentiated tumours, respectively (P<0.05). In addition, MAGE 
      immunoreactivity was significantly correlated with lymphatic vessel invasion and 
      intratumoural necrosis. Moreover, a significant inverse relationship with 
      oestrogen receptor expression was also observed. However, no significant 
      correlation could be established between MAGE immunoreactivity and defined 
      phenotypic characteristics of tumour infiltrating lymphocytes, including 
      expression of CD3, CD4, CD8, CD20 or granzyme B. Thus, expression of MAGE family 
      gene products in invasive ductal breast cancers appears to be associated with 
      poorly differentiated histological phenotypes. These data support the concept of 
      specific immunotherapy in highly aggressive forms of breast neoplasms. 
      Furthermore, they suggest that MAGE immunoreactivity could represent a tumour 
      marker of potential prognostic relevance.
FAU - Kavalar, R
AU  - Kavalar R
AD  - Maribor General Hospital, Slovenia.
FAU - Sarcevic, B
AU  - Sarcevic B
FAU - Spagnoli, G C
AU  - Spagnoli GC
FAU - Separovic, V
AU  - Separovic V
FAU - Samija, M
AU  - Samija M
FAU - Terracciano, L
AU  - Terracciano L
FAU - Heberer, M
AU  - Heberer M
FAU - Juretic, A
AU  - Juretic A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Melanoma-Specific Antigens)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Neoplasm
MH  - Biomarkers, Tumor/analysis
MH  - Breast Neoplasms/classification/*metabolism/pathology
MH  - Carcinoma, Ductal, Breast/classification/*metabolism/secondary
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Transformation, Neoplastic
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Lymph Nodes/metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Melanoma-Specific Antigens
MH  - Neoplasm Invasiveness/pathology
MH  - Neoplasm Proteins/immunology/*metabolism
EDAT- 2001/09/20 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/09/20 10:00
PHST- 2001/09/20 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/09/20 10:00 [entrez]
AID - 10.1007/s004280100421 [doi]
PST - ppublish
SO  - Virchows Arch. 2001 Aug;439(2):127-31. doi: 10.1007/s004280100421.