PMID- 11562534
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20200217
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 82
IP  - Pt 10
DP  - 2001 Oct
TI  - Human cytomegalovirus binding to heparan sulfate proteoglycans on the cell 
      surface and/or entry stimulates the expression of human leukocyte antigen class 
      I.
PG  - 2405-2413
LID - 10.1099/0022-1317-82-10-2405 [doi]
AB  - Human cytomegalovirus (HCMV) is known to down-regulate the expression of human 
      leukocyte antigen (HLA) class I, the process of which involves a subset of virus 
      genes. Infection of human foreskin fibroblast (HFF) cells with UV-inactivated 
      HCMV (UV-HCMV), however, resulted in an increase in HLA class I presentation on 
      the cell surface in the absence of HCMV gene expression. Heparin, which inhibits 
      the interaction of virus particles with cell surface heparan sulfate 
      proteoglycans (HSPGs), blocked the effect of UV-HCMV on HLA class I expression. 
      Pretreatment of cells with heparinase I decreased in a dose-dependent manner the 
      effect of UV-HCMV on HLA class I expression enhancement. Sodium chlorate, which 
      is known to inhibit the sulfation of HSPGs, gave a similar result. Pretreatment 
      of UV-HCMV with trypsin or monoclonal antibody reactive with the envelope 
      glycoprotein gB reduced the increase in HLA class I expression on the HFF cell 
      surface by UV-HCMV. RT-PCR analysis demonstrated that the increase in HLA class I 
      presentation on the HFF cell surface was due to an increase in HLA class I 
      transcription. Thus, binding of HCMV particles to cell surface HSPGs appears to 
      be required for the stimulation of HLA class I expression. It is also possible 
      that virus entry, in addition to binding to HSPGs, may be involved in the 
      stimulation of HLA class I expression, since the UV-HCMV entered the cells and 
      all treatments to block virus binding to HSPGs would necessarily prevent virus 
      entry.
FAU - Song, Byung H
AU  - Song BH
AD  - Division of Life Sciences, College of Natural Sciences1 and Research Institute 
      for Genetic Engineering2, Chungbuk National University, Cheongju, Chungbuk 
      361-763, South Korea.
FAU - Lee, Gyu C
AU  - Lee GC
AD  - Division of Life Sciences, College of Natural Sciences1 and Research Institute 
      for Genetic Engineering2, Chungbuk National University, Cheongju, Chungbuk 
      361-763, South Korea.
FAU - Moon, Myung S
AU  - Moon MS
AD  - Division of Life Sciences, College of Natural Sciences1 and Research Institute 
      for Genetic Engineering2, Chungbuk National University, Cheongju, Chungbuk 
      361-763, South Korea.
FAU - Cho, Yeon H
AU  - Cho YH
AD  - Division of Life Sciences, College of Natural Sciences1 and Research Institute 
      for Genetic Engineering2, Chungbuk National University, Cheongju, Chungbuk 
      361-763, South Korea.
FAU - Lee, Chan H
AU  - Lee CH
AD  - Division of Life Sciences, College of Natural Sciences1 and Research Institute 
      for Genetic Engineering2, Chungbuk National University, Cheongju, Chungbuk 
      361-763, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Heparan Sulfate Proteoglycans)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein B, Simplexvirus)
SB  - IM
MH  - Cells, Cultured
MH  - Cytomegalovirus/immunology/*physiology
MH  - Heparan Sulfate Proteoglycans/*physiology
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Transcription, Genetic
MH  - Viral Envelope Proteins/physiology
MH  - Viral Vaccines/immunology
EDAT- 2001/09/20 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/09/20 10:00
PHST- 2001/09/20 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/09/20 10:00 [entrez]
AID - 10.1099/0022-1317-82-10-2405 [doi]
PST - ppublish
SO  - J Gen Virol. 2001 Oct;82(Pt 10):2405-2413. doi: 10.1099/0022-1317-82-10-2405.