PMID- 11564064
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 114
IP  - 4
DP  - 2001 Sep
TI  - Interphase fluorescence in situ hybridization and spectral karyotyping reveals 
      hidden genetic aberrations in children with acute lymphoblastic leukaemia and a 
      normal banded karyotype.
PG  - 786-93
AB  - Twenty-two cases of childhood acute lymphoblastic leukaemia (ALL) with normal G- 
      or Q-banded karyotypes were studied by interphase fluorescence in situ 
      hybridization (FISH) and spectral karyotyping. Probes detecting MLL, BCR/ABL and 
      TEL/AML1 rearrangements were used for the interphase studies, along with 
      centromere-specific probes from chromosomes 17 and X. In 10 patients (45%), 
      previously undetected aberrations were demonstrable. Specific gene rearrangements 
      and structural changes were found in six cases and numerical changes in five. 
      Five of these aberrations have previously been reported to have an impact on 
      prognosis. Three cases were massively hyperdiploid and, in one, the 
      prognostically important BCR/ABL fusion was detected. In addition, a near-haploid 
      karyotype with 27 chromosomes was found in one patient and TEL/AML1 
      rearrangements were detected in two cases. This study indicates that about half 
      of childhood ALL cases with apparently normal karyotypes harbour genetic 
      aberrations that may be detected using interphase FISH and spectral karyotyping.
FAU - Nordgren, A
AU  - Nordgren A
AD  - Department of Molecular Medicine, L8-02 Karolinska Hospital, SE-171 76 Stockholm, 
      Sweden. ann.nordgren@cmm.ki.se
FAU - Schoumans, J
AU  - Schoumans J
FAU - Soderhall, S
AU  - Soderhall S
FAU - Nordenskjold, M
AU  - Nordenskjold M
FAU - Blennow, E
AU  - Blennow E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - Chromosomes, Human, Pair 17
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase
MH  - Karyotyping/methods
MH  - Leukemia-Lymphoma, Adult T-Cell/genetics
MH  - Male
MH  - Oncogene Proteins, Fusion/genetics
MH  - Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - X Chromosome
EDAT- 2001/09/21 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/21 10:00
PHST- 2001/09/21 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/21 10:00 [entrez]
AID - bjh3008 [pii]
AID - 10.1046/j.1365-2141.2001.03008.x [doi]
PST - ppublish
SO  - Br J Haematol. 2001 Sep;114(4):786-93. doi: 10.1046/j.1365-2141.2001.03008.x.