PMID- 11564600 OWN - NLM STAT- MEDLINE DCOM- 20011207 LR - 20231213 IS - 0952-5041 (Print) IS - 0952-5041 (Linking) VI - 27 IP - 2 DP - 2001 Oct TI - A spontaneously arising mutation in connexin32 with repeated passage of FRTL-5 cells coincides with increased growth rate and reduced thyroxine release. PG - 145-63 AB - In this study we examine changes in the cellular properties of FRTL-5 cells as a function of passage number, with particular emphasis on gap junction expression, karyotype, morphology, growth rate and thyroxine (T(4)) release. Early passage FRTL-5 follicular cells transfer dye through gap junctions from injected cell(s) to third-order neighboring cells and beyond within their respective follicles and have immuno-detectable connexin32 (Cx32) type gap junctional plaques in their lateral contacting plasma membranes. By contrast, FRTL-5 cells established as monolayers, or as follicles from cultures passed more than 15 times, did not transfer microinjected Lucifer Yellow dye to contiguous neighboring cells and did not express any immuno-detectable rat thyroid specific connexins (Cx43, Cx32 or Cx26). Western blots confirmed that total, membrane and cytosolic Cx32 protein was present only in early pass follicular cultures. To better understand the passage-dependent loss of Cx32 expression, RT-PCR primers were made to the most unique sequences of the rat Cx32 molecule, the cytoplasmic and carboxyl-terminal regions. These primers were used to screen FRTL-5 RNA from cultures of various passage numbers. The results revealed that later passage cultures had a single base deletion in the middle of the Cx32 cytoplasmic loop region at nucleotide position 378. This base deletion was in the middle position of the codon for amino acid 116, which is normally a CAC (histidine) but read with the frame shift was a CCC (proline). The four amino acids that followed this deletion were also altered with the fourth one becoming UAA, the ochre translation stop codon. This premature stopping of translation resulted in a truncation of 60% of the protein, which included the remaining cytoplasmic loop, third and fourth transmembrane regions and the carboxyl-terminus. The later passage cultures did not produce a carboxyl-terminal RT-PCR product, indicating that the mRNA was also truncated. These regions of the Cx32 molecule contain the sequences and epitopes to which probes and antibodies are directed, and as such alterations of these regions with repeated passage explains reports by others that FRTL-5 cells do not express Cx32, and implies that cultures used for these assessments were passed more than 15 times. To determine if genetic or epigenetic abnormalities existed in FRTL-5 cells we performed chromosome spreads from various passage cultures. FRTL-5 cells have been reported to be diploid and more recently non-diploid; however, we found them to be fully tetraploid. This tetraploidy appears to be unstable in that later passes are tetraploid plus two or three extra chromosomes. There were no obvious translocations, breaks or large-scale interstitial deletions of any chromosomes in the FRTL-5 cultures tested. As FRTL-5 cells were repeatedly passed their morphology changed. Monolayer areas spread from beneath the follicles, and the follicles became flattened in appearance. These physical changes were coincident with dramatically increased growth rates. Early cultures (passed 3-12 times) divided on average every 49+/-1 h, whereas later passes (passes 20-25) divided every 28+/-3 h. To correlate these changes with a measure of thyroid function we assayed T(4) output. Early passage follicular cultures incubated for 6 h with sodium iodide, released on average 5.27+/- 0.33 ng/ml of T(4)/100 follicles. Later passes, or early passes treated with heptanol to down-regulate Cx32, released an average of 3.84+/-0.50 ng/ml of T(4)/100 follicles. There was a 27% difference in T(4) release between early follicular cultures, that were coupled by Cx32, and late or down-regulated early follicular cultures, that were uncoupled (P<0.0001). Collectively, the physical changes documented in this study were coincident with the loss of functional Cx32. This implies a relationship between the loss of intercellular communication and changes in morphogenic appearance, growth rate and reduced thyroid function and supports the previously postulated, tumor-suppressor role for Cx32. FRTL-5 cultures from low passage numbers are an excellent model of primary thyroid cells. However, many reports in the literature ascribe features to FRTL-5 cells that are mutually inconsistent. These differences may be resolved in the future by addressing the passage number and the conditional differences of the cultures being studied. FAU - Green, L M AU - Green LM AD - Radiobiology Program, Department of Radiation Medicine, Chan Shun Pavilion, Room A-101, Loma Linda University, 11175 Campus Street, Loma Linda, California 92354, USA. lgreen@dominion.llumc.edu FAU - Murray, D K AU - Murray DK FAU - Tran, D T AU - Tran DT FAU - Nelson, G A AU - Nelson GA FAU - Shah, M M AU - Shah MM FAU - Luben, R A AU - Luben RA LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PL - England TA - J Mol Endocrinol JT - Journal of molecular endocrinology JID - 8902617 RN - 0 (Connexins) RN - 0 (RNA, Messenger) RN - 9007-49-2 (DNA) RN - Q51BO43MG4 (Thyroxine) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cell Communication MH - Cell Culture Techniques MH - Cell Division MH - Cell Line MH - Connexins/*genetics/metabolism MH - DNA/genetics MH - Gap Junctions/physiology MH - Gene Expression MH - Karyotyping MH - Molecular Sequence Data MH - *Mutation MH - Point Mutation MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Sequence Deletion MH - Thyroid Gland/cytology/metabolism MH - Thyroxine/metabolism MH - Gap Junction beta-1 Protein OTO - NASA OT - NASA Discipline Radiation Health OT - Non-NASA Center FIR - Nelson, G A IR - Nelson GA IRAD- Loma Linda U, CA EDAT- 2001/09/21 10:00 MHDA- 2002/01/05 10:01 CRDT- 2001/09/21 10:00 PHST- 2001/09/21 10:00 [pubmed] PHST- 2002/01/05 10:01 [medline] PHST- 2001/09/21 10:00 [entrez] AID - JME01031 [pii] AID - 10.1677/jme.0.0270145 [doi] PST - ppublish SO - J Mol Endocrinol. 2001 Oct;27(2):145-63. doi: 10.1677/jme.0.0270145.