PMID- 11566346
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 129
IP  - 2
DP  - 2001 Sep
TI  - Localization of the chromosome 22 breakpoints in two cases of acute 
      megakaryoblastic leukemia with t(1;22)(p13;q13).
PG  - 150-4
AB  - Acute megakaryoblastic leukemia with t(1;22)(p13;q13) is a rare malignancy 
      occurring in infants and young children. The genes involved in t(1;22)(p13;q13) 
      are unknown. In this study, dual-color fluorescence in situ hybridization (FISH) 
      experiments with 15 probes were performed on the metaphase cells obtained from 
      one patient to systematically narrow the region of the breakpoint on chromosome 
      22 and localize it to RP5-1042K10. A 22.3-kb FISH probe derived from RP5-1042K10 
      was used to further refine the locus of the breakpoint in this case. Southern 
      blot analysis covering of genomic DNA from a second patient detected DNA 
      rearrangement at a site close to the breakpoint observed with the 22.3-kb probe 
      in the first case. A partially characterized gene, KIAA 1438, is in the vicinity 
      of the breakpoints determined by FISH and Southern blot experiments, suggesting 
      that this gene plays a role in this malignancy.
FAU - Dickstein, J I
AU  - Dickstein JI
AD  - Department of Pathology, MC008, University of Chicago, 5841 S. Maryland Avenue, 
      Chicago, IL 60637, USA. jdickste@mcis.bsd.uchicago.edu
FAU - Davis, E M
AU  - Davis EM
FAU - Roulston, D
AU  - Roulston D
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Blotting, Southern
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Fatal Outcome
MH  - Female
MH  - Genes, Neoplasm
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Leukemia, Megakaryoblastic, Acute/diagnosis/*genetics
MH  - Male
MH  - Metaphase
MH  - Translocation, Genetic/*genetics
EDAT- 2001/09/22 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/09/22 10:00
PHST- 2001/09/22 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/09/22 10:00 [entrez]
AID - S0165-4608(01)00508-8 [pii]
AID - 10.1016/s0165-4608(01)00508-8 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Sep;129(2):150-4. doi: 
      10.1016/s0165-4608(01)00508-8.