PMID- 11566351
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 129
IP  - 2
DP  - 2001 Sep
TI  - Routine fluorescence in situ hybridization with the MLL probe does not reliably 
      detect two separate signals on one chromosome 11 in patients with trisomy 11.
PG  - 173-6
AB  - Trisomy 11 is considered to be a rare cytogenetic abnormality in myelodysplastic 
      syndromes (MDS) and acute myelogenous leukemia (AML). Duplication of the MLL gene 
      (localized to 11q23) has been found on one chromosome 11 in patients with trisomy 
      11, detected by DNA techniques. We investigated copy number of MLL in seven 
      patients with trisomy 11 to see if duplication could be assessed by the detection 
      of two separate signals on fluorescence in situ hybridization (FISH). If so, FISH 
      could provide a quick easy screen of MLL status in routine referrals. The 
      diagnostic bone marrow aspirate showed trisomy 11 in five adult patients with 
      MDS/AML as part of a complex karyotype and in two children with acute 
      lymphoblastic leukemia (ALL) as part of a hyperdiploid karyotype. Fluorescence in 
      situ hybridization utilized the suspensions remaining after the cytogenetic 
      harvest. Two FISH probes were used on the adult patients (MLL - Oncor and Vysis), 
      and one (Vysis) for the two children with ALL. Analysis showed that the proximity 
      of the two putative hybridization signals made it very difficult to unambiguously 
      see two separate signals. The hybridisations (Oncor probe) were convincing of MLL 
      duplication (namely two distinct signals) in only one patient, but this was not 
      borne out with the other MLL probe (Vysis). We conclude that conventional FISH 
      with MLL probe is not suited to act as a screen for MLL duplication in patients 
      with trisomy 11.
FAU - Smith, A
AU  - Smith A
AD  - Department of Cytogenetics, Royal Alexandra Hospital for Children, Locked Bag 
      4001, NSW 2145, Westmead, Australia. ellies@nch.edu.au
FAU - Robson, L
AU  - Robson L
FAU - Heaps, L S
AU  - Heaps LS
FAU - Sharma, P
AU  - Sharma P
FAU - Dunlop, L
AU  - Dunlop L
FAU - Bhave, A
AU  - Bhave A
FAU - Bradstock, K
AU  - Bradstock K
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 11/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/diagnosis/*genetics
MH  - Myelodysplastic Syndromes/diagnosis/*genetics
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - *Nucleic Acid Probes
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*genetics
MH  - Predictive Value of Tests
MH  - *Proto-Oncogenes
MH  - Sensitivity and Specificity
MH  - *Transcription Factors
MH  - Trisomy/diagnosis/*genetics
EDAT- 2001/09/22 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/09/22 10:00
PHST- 2001/09/22 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/09/22 10:00 [entrez]
AID - S0165-4608(01)00449-6 [pii]
AID - 10.1016/s0165-4608(01)00449-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Sep;129(2):173-6. doi: 
      10.1016/s0165-4608(01)00449-6.