PMID- 11568088
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 47
IP  - 10
DP  - 2001 Oct
TI  - Usefulness of saliva for measurement of 3,4-methylenedioxymethamphetamine and its 
      metabolites: correlation with plasma drug concentrations and effect of salivary 
      pH.
PG  - 1788-95
AB  - BACKGROUND: Saliva is an alternative biologic matrix for drugs-of-abuse testing 
      that offers the advantages of noninvasive, rapid, and easy sampling. We studied 
      the excretion profile of 3,4-methylenedioxymethamphetamine (MDMA) and its 
      metabolites in both saliva and plasma, as well the effect of the drug on salivary 
      pH. METHODS: Saliva and plasma samples were obtained from eight healthy MDMA 
      consumers after ingestion of a single 100-mg dose of the drug. Concentrations of 
      MDMA and its main metabolites, 3,4-methylenedioxyamphetamine (MDA) and 
      4-hydroxy-3-methoxymethamphetamine (HMMA), in saliva and plasma were measured by 
      gas chromatography-mass spectrometry. Apparent pharmacokinetic parameters for 
      MDMA in saliva were estimated, and the saliva-to-plasma ratio at each time 
      interval was calculated and correlated with salivary pH. RESULTS: MDMA, MDA, and 
      HMMA were detected in saliva. Salivary concentrations of MDMA were 1728.9-6510.6 
      microg/L and peaked at 1.5 h after drug intake. This was followed by a 
      progressive decrease, with a mean concentration of 126.2 microg/L at 24 h. The 
      saliva-to-plasma ratio was 32.3-1.2, with a peak of 18.1 at 1.5 h after drug 
      administration. Salivary pH seemed to be affected by MDMA administration; pH 
      values decreased by 0.6 units (mean pH values of 6.9 and 6.8 at 1.5 and 4 h after 
      drug administration vs predose pH of 7.4). CONCLUSIONS: Measurement of MDMA in 
      saliva is a valuable alternative to determination of plasma drug concentrations 
      in both clinical and toxicologic studies. On-site testing is also facilitated by 
      noninvasive and rapid collection of salivary specimens.
FAU - Navarro, M
AU  - Navarro M
AD  - Department of Pharmacology, Institut Municipal d'Investigacio Meedica (IMIM), 
      E-08003 Barcelona, Spain.
FAU - Pichini, S
AU  - Pichini S
FAU - Farre, M
AU  - Farre M
FAU - Ortuno, J
AU  - Ortuno J
FAU - Roset, P N
AU  - Roset PN
FAU - Segura, J
AU  - Segura J
FAU - de la Torre, R
AU  - de la Torre R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Lactates)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - 515-30-0 (atrolactic acid)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analysis/blood
MH  - Adult
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lactates/analysis/blood
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis/blood/pharmacokinetics
MH  - Saliva/*chemistry/metabolism
MH  - Substance Abuse Detection/*methods
EDAT- 2001/09/25 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/09/25 10:00
PHST- 2001/09/25 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/09/25 10:00 [entrez]
PST - ppublish
SO  - Clin Chem. 2001 Oct;47(10):1788-95.