PMID- 11569807
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 73
IP  - 17
DP  - 2001 Sep 1
TI  - Surface biopassivation of replicated poly(dimethylsiloxane) microfluidic channels 
      and application to heterogeneous immunoreaction with on-chip fluorescence 
      detection.
PG  - 4181-9
AB  - Poly(dimethylsiloxane) (PDMS) appeared recently as a material of choice for rapid 
      and accurate replication of polymer-based microfluidic networks. However, due to 
      its hydrophobicity, the surface strongly interacts with apolar analytes or 
      species containing apolar domains, resulting in significant uncontrolled 
      adsorption on channel walls. This contribution describes the application and 
      characterization of a PDMS surface treatment that considerably decreases 
      adsorption of low and high molecular mass substances to channel walls while 
      maintaining a modest cathodic electroosmotic flow. Channels are modified with a 
      three-layer biotin-neutravidin sandwich coating, made of biotinylated IgG, 
      neutravidin, and biotinylated dextran. By replacing biotinylated dextran with any 
      biotinylated reagent, the modified surface can be readily patterned with 
      biochemical probes, such as antibodies. Combination of probe immobilization 
      chemistry with low nonspecific binding enables affinity binding assays within 
      channel networks. The example of an electrokinetic driven, heterogeneous 
      immunoreaction for human IgG is described.
FAU - Linder, V
AU  - Linder V
AD  - Centre Suisse d'Electronique et de Microtechnique, Neuchatel.
FAU - Verpoorte, E
AU  - Verpoorte E
FAU - Thormann, W
AU  - Thormann W
FAU - de Rooij, N F
AU  - de Rooij NF
FAU - Sigrist, H
AU  - Sigrist H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Humans
MH  - Immunochemistry/*instrumentation
MH  - Immunoglobulin G/analysis
MH  - Microcomputers
MH  - Spectrometry, Fluorescence/*instrumentation
EDAT- 2001/09/25 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/09/25 10:00
PHST- 2001/09/25 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/09/25 10:00 [entrez]
AID - 10.1021/ac010421e [doi]
PST - ppublish
SO  - Anal Chem. 2001 Sep 1;73(17):4181-9. doi: 10.1021/ac010421e.