PMID- 11571165
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 67
IP  - 10
DP  - 2001 Oct
TI  - Relationships between microbial community structure and hydrochemistry in a 
      landfill leachate-polluted aquifer.
PG  - 4619-29
AB  - Knowledge about the relationship between microbial community structure and 
      hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill 
      leachate-polluted aquifers is required to develop tools for predicting and 
      monitoring natural attenuation. In this study analyses of pollutant and redox 
      chemistry were conducted in parallel with culture-independent profiling of 
      microbial communities present in a well-defined aquifer (Banisveld, The 
      Netherlands). Degradation of organic contaminants occurred under iron-reducing 
      conditions in the plume of pollution, while upstream of the landfill and above 
      the plume denitrification was the dominant redox process. Beneath the plume iron 
      reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based 
      denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea 
      in 29 groundwater samples revealed a clear difference between the microbial 
      community structures inside and outside the contaminant plume. A similar 
      relationship was not evident in sediment samples. DGGE data were supported by 
      sequencing cloned 16S rDNA. Upstream of the landfill members of the beta subclass 
      of the class Proteobacteria (beta-proteobacteria) dominated. This group was not 
      encountered beneath the landfill, where gram-positive bacteria dominated. Further 
      downstream the contribution of gram-positive bacteria to the clone library 
      decreased, while the contribution of delta-proteobacteria strongly increased and 
      beta-proteobacteria reappeared. The beta-proteobacteria (Acidovorax, Rhodoferax) 
      differed considerably from those found upstream (Gallionella, Azoarcus). Direct 
      comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data 
      from each analysis were comparable. A relationship was observed between the 
      dominant redox processes and the bacteria identified. In the iron-reducing plume 
      members of the family Geobacteraceae made a strong contribution to the microbial 
      communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing 
      bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated 
      aquifers deserves more detailed consideration.
FAU - Roling, W F
AU  - Roling WF
AD  - Section of Molecular Microbial Ecology, Department of Molecular Cell Physiology, 
      Faculty of Biology, Research School SENSE, Vrije Universiteit, NL-1081 HV 
      Amsterdam, The Netherlands.
FAU - van Breukelen, B M
AU  - van Breukelen BM
FAU - Braster, M
AU  - Braster M
FAU - Lin, B
AU  - Lin B
FAU - van Verseveld, H W
AU  - van Verseveld HW
LA  - eng
SI  - GENBANK/AY013585
SI  - GENBANK/AY013586
SI  - GENBANK/AY013587
SI  - GENBANK/AY013588
SI  - GENBANK/AY013589
SI  - GENBANK/AY013590
SI  - GENBANK/AY013591
SI  - GENBANK/AY013592
SI  - GENBANK/AY013593
SI  - GENBANK/AY013594
SI  - GENBANK/AY013595
SI  - GENBANK/AY013596
SI  - GENBANK/AY013597
SI  - GENBANK/AY013598
SI  - GENBANK/AY013599
SI  - GENBANK/AY013600
SI  - GENBANK/AY013601
SI  - GENBANK/AY013602
SI  - GENBANK/AY013603
SI  - GENBANK/AY013604
SI  - GENBANK/AY013605
SI  - GENBANK/AY013606
SI  - GENBANK/AY013607
SI  - GENBANK/AY013608
SI  - GENBANK/AY013609
SI  - GENBANK/AY013610
SI  - GENBANK/AY013611
SI  - GENBANK/AY013612
SI  - GENBANK/AY013613
SI  - GENBANK/AY013614
SI  - GENBANK/AY013615
SI  - GENBANK/AY013616
SI  - GENBANK/AY013617
SI  - GENBANK/AY013618
SI  - GENBANK/AY013619
SI  - GENBANK/AY013620
SI  - GENBANK/AY013621
SI  - GENBANK/AY013622
SI  - GENBANK/AY013623
SI  - GENBANK/AY013624
SI  - GENBANK/AY013625
SI  - GENBANK/AY013626
SI  - GENBANK/AY013627
SI  - GENBANK/AY013628
SI  - GENBANK/AY013629
SI  - GENBANK/AY013630
SI  - GENBANK/AY013631
SI  - GENBANK/AY013632
SI  - GENBANK/AY013633
SI  - GENBANK/AY013634
SI  - GENBANK/AY013635
SI  - GENBANK/AY013636
SI  - GENBANK/AY013637
SI  - GENBANK/AY013638
SI  - GENBANK/AY013639
SI  - GENBANK/AY013640
SI  - GENBANK/AY013641
SI  - GENBANK/AY013642
SI  - GENBANK/AY013643
SI  - GENBANK/AY013644
SI  - GENBANK/AY013645
SI  - GENBANK/AY013646
SI  - GENBANK/AY013647
SI  - GENBANK/AY013648
SI  - GENBANK/AY013649
SI  - GENBANK/AY013650
SI  - GENBANK/AY013651
SI  - GENBANK/AY013652
SI  - GENBANK/AY013653
SI  - GENBANK/AY013654
SI  - GENBANK/AY013655
SI  - GENBANK/AY013656
SI  - GENBANK/AY013657
SI  - GENBANK/AY013658
SI  - GENBANK/AY013660
SI  - GENBANK/AY013661
SI  - GENBANK/AY013662
SI  - GENBANK/AY013663
SI  - GENBANK/AY013664
SI  - GENBANK/AY013665
SI  - GENBANK/AY013666
SI  - GENBANK/AY013667
SI  - GENBANK/AY013668
SI  - GENBANK/AY013669
SI  - GENBANK/AY013670
SI  - GENBANK/AY013671
SI  - GENBANK/AY013672
SI  - GENBANK/AY013673
SI  - GENBANK/AY013674
SI  - GENBANK/AY013675
SI  - GENBANK/AY013676
SI  - GENBANK/AY013677
SI  - GENBANK/AY013678
SI  - GENBANK/AY013679
SI  - GENBANK/AY013680
SI  - GENBANK/AY013681
SI  - GENBANK/AY013682
SI  - GENBANK/AY013683
SI  - GENBANK/AY013684
SI  - GENBANK/AY013685
SI  - GENBANK/AY013686
SI  - GENBANK/AY013687
SI  - GENBANK/AY013688
SI  - GENBANK/AY013689
SI  - GENBANK/AY013690
SI  - GENBANK/AY013691
SI  - GENBANK/AY013692
SI  - GENBANK/AY013693
SI  - GENBANK/AY013694
SI  - GENBANK/AY013695
SI  - GENBANK/AY013696
SI  - GENBANK/AY013697
SI  - GENBANK/AY013698
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Archaea/classification/genetics
MH  - Bacteria/classification/genetics
MH  - Cloning, Molecular
MH  - DNA, Ribosomal/analysis
MH  - *Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Fresh Water/*chemistry/*microbiology
MH  - Geologic Sediments/chemistry/microbiology
MH  - Molecular Sequence Data
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Refuse Disposal
MH  - Sequence Analysis, DNA
MH  - *Water Pollution, Chemical
PMC - PMC93212
EDAT- 2001/09/26 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/10/01
CRDT- 2001/09/26 10:00
PHST- 2001/09/26 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/26 10:00 [entrez]
PHST- 2001/10/01 00:00 [pmc-release]
AID - 0152 [pii]
AID - 10.1128/AEM.67.10.4619-4629.2001 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2001 Oct;67(10):4619-29. doi: 
      10.1128/AEM.67.10.4619-4629.2001.