PMID- 11571193
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 67
IP  - 10
DP  - 2001 Oct
TI  - Detection and enumeration of methanotrophs in acidic Sphagnum peat by 16S rRNA 
      fluorescence in situ hybridization, including the use of newly developed 
      oligonucleotide probes for Methylocella palustris.
PG  - 4850-7
AB  - Two 16S rRNA-targeted oligonucleotide probes, Mcell-1026 and Mcell-181, were 
      developed for specific detection of the acidophilic methanotroph Methylocella 
      palustris using fluorescence in situ hybridization (FISH). The fluorescence 
      signal of probe Mcell-181 was enhanced by its combined application with the 
      oligonucleotide helper probe H158. Mcell-1026 and Mcell-181, as well as 16S rRNA 
      oligonucleotide probes with reported group specificity for either type I 
      methanotrophs (probes M-84 and M-705) or the Methylosinus/Methylocystis group of 
      type II methanotrophs (probes MA-221 and M-450), were used in FISH to determine 
      the abundance of distinct methanotroph groups in a Sphagnum peat sample of pH 
      4.2. M. palustris was enumerated at greater than 10(6) cells per g of peat (wet 
      weight), while the detectable population size of type I methanotrophs was three 
      orders of magnitude below the population level of M. palustris. The cell counts 
      with probe MA-221 suggested that only 10(4) type II methanotrophs per g of peat 
      (wet weight) were present, while the use of probe M-450 revealed more than 10(6) 
      type II methanotroph cells per g of the same samples. This discrepancy was due to 
      the fact that probe M-450 targets almost all currently known strains of 
      Methylosinus and Methylocystis, whereas probe MA-221, originally described as 
      group specific, does not detect a large proportion of Methylocystis strains. The 
      total number of methanotrophic bacteria detected by FISH was 3.0 (+/-0.2) x 10(6) 
      cells per g (wet weight) of peat. This was about 0.8% of the total bacterial cell 
      number. Thus, our study clearly suggests that M. palustris and a defined 
      population of Methylocystis spp. were the predominant methanotrophs detectable by 
      FISH in an acidic Sphagnum peat bog.
FAU - Dedysh, S N
AU  - Dedysh SN
AD  - Institute of Microbiology, Russian Academy of Sciences, Moscow 117811, Russia.
FAU - Derakshani, M
AU  - Derakshani M
FAU - Liesack, W
AU  - Liesack W
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Alphaproteobacteria/classification/genetics/*isolation & purification
MH  - Bryopsida/*microbiology
MH  - Colony Count, Microbial
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Methane/*metabolism
MH  - Methylococcaceae/classification/genetics/*isolation & purification
MH  - Oligonucleotide Probes/genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - *Soil Microbiology
MH  - Species Specificity
PMC - PMC93240
EDAT- 2001/09/26 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/10/01
CRDT- 2001/09/26 10:00
PHST- 2001/09/26 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/26 10:00 [entrez]
PHST- 2001/10/01 00:00 [pmc-release]
AID - 0421 [pii]
AID - 10.1128/AEM.67.10.4850-4857.2001 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2001 Oct;67(10):4850-7. doi: 
      10.1128/AEM.67.10.4850-4857.2001.