PMID- 11571710
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20071114
IS  - 1084-8592 (Print)
IS  - 1084-8592 (Linking)
VI  - 6
IP  - 3
DP  - 2001 Sep
TI  - Detection of clonal T-cell receptor gamma gene rearrangements using 
      fluorescent-based PCR and automated high-resolution capillary electrophoresis.
PG  - 169-79
AB  - BACKGROUND: Analysis of T-cell receptor gamma (TCR gamma) gene rearrangements by 
      PCR is a powerful tool for detecting clonal T-cell populations for the diagnosis 
      of lymphoid neoplasms. We report a method for TCR gamma PCR analysis using 
      capillary electrophoresis (CE). METHODS AND RESULTS: To define the threshold for 
      identification of a predominant monoclonal population within a polyclonal 
      background, we developed a novel objective parameter of the peak height ratio 
      (Rn) of the peak of interest and the average of the two immediate flanking peaks. 
      After evaluation of monoclonal, reactive, and normal T-cell populations, an Rn of 
      3.0 or greater was determined to be consistent with a monoclonal population, 
      whereas an Rn between 1.9 and 3.0 was considered an intermediate range. This CE 
      method was compared with the standard denaturing gradient gel electrophoresis 
      (DGGE) method using previously evaluated clinical specimens. Eleven of 12 
      clinical specimens (92%) with a definitive diagnosis of T-cell lymphoma were 
      monoclonal by CE, with 100% concordance with the DGGE method. Of nine specimens 
      morphologically suspicious for T-cell lymphoma, five specimens were positive by 
      CE analysis compared with four specimens by DGGE. In addition, 14 specimens for 
      staging from patients with known T-cell lymphoma were studied using both the CE 
      and DGGE methods, with a concordance of 86%. CONCLUSION: CE is a powerful and 
      efficient method for analysis of clonality by TCR gamma PCR.
FAU - Luo, V
AU  - Luo V
AD  - Molecular Pathology Laboratory, Department of Pathology and Laboratory Medicine, 
      University of Pennsylvania, Philadelphia, PA 19104-4283, USA.
FAU - Lessin, S R
AU  - Lessin SR
FAU - Wilson, R B
AU  - Wilson RB
FAU - Rennert, H
AU  - Rennert H
FAU - Tozer, C
AU  - Tozer C
FAU - Benoit, B
AU  - Benoit B
FAU - Leonard, D G
AU  - Leonard DG
LA  - eng
GR  - K44 AR02102/AR/NIAMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Diagn
JT  - Molecular diagnosis : a journal devoted to the understanding of human disease 
      through the clinical application of molecular biology
JID - 9614965
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Biopsy
MH  - Clone Cells
MH  - DNA Primers
MH  - DNA, Neoplasm/analysis
MH  - Electrophoresis, Capillary
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fluorescence
MH  - Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor/*genetics
MH  - Genes, T-Cell Receptor gamma/*genetics
MH  - Humans
MH  - Lymphoma, T-Cell/genetics/pathology
MH  - Lymphoma, T-Cell, Cutaneous/*genetics/pathology
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*genetics/pathology
EDAT- 2001/09/26 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/09/26 10:00
PHST- 2001/09/26 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/09/26 10:00 [entrez]
AID - S1084-8592(01)67888-3 [pii]
AID - 10.1054/modi.2001.27056 [doi]
PST - ppublish
SO  - Mol Diagn. 2001 Sep;6(3):169-79. doi: 10.1054/modi.2001.27056.