PMID- 11574821
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20131121
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 138
IP  - 4
DP  - 2001 Oct
TI  - Role of nitric oxide in acidosis-induced intestinal injury in anesthetized rats.
PG  - 270-6
AB  - We investigated the pathogenic mechanism(s) of small intestinal injury during 
      acidosis in relation to circulating nitric oxide (NO) in an experimental rat 
      model. Rats were anesthetized, paralyzed, and mechanically ventilated with room 
      air. Hydrochloric acid (0.16 mmol bolus followed by 0.132 mmol/kg/h) was infused 
      through the jugular vein for 5 hours. Control rats received a saline infusion. 
      Arterial blood gases, blood pressure, and blood pH were measured every 30 
      minutes. The involvement of NO in this acidosis model was assessed by measuring 
      plasma concentration of nitrite/nitrate (NOx) and by evaluating inducible NO 
      synthase (iNOS) expression in small intestinal mucosa. Intestinal injury was 
      assessed by measuring myeloperoxidase (MPO) activity, thiobarbituric acid 
      reactants (TBARS), and histologic scores. HCl infusion was associated with 
      hypotension, decreased blood pH, increased plasma concentration of NOx, augmented 
      intestinal mucosal iNOS expression, MPO activity, TBARS, and histopathologic 
      injury scores. Pretreatment with an iNOS inhibitor, aminoguanidine (AG, 50 
      mg/kg), reversed HCl-induced hypotension without a change in blood pH. 
      HCl-induced lesions, MPO activity, TBARS, and plasma NOx production were 
      decreased by AG. Our data show that the pathogenic mechanisms of acidosis-induced 
      small intestinal lesions involve up-regulation of NO production by increased 
      expression of iNOS and augmentation of superoxide radicals and MPO activity.
FAU - Pedoto, A
AU  - Pedoto A
AD  - Department of Anesthesiology, State University of New York, Upstate Medical 
      University, Syracuse, NY 13210, USA.
FAU - Nandi, J
AU  - Nandi J
FAU - Oler, A
AU  - Oler A
FAU - Camporesi, E M
AU  - Camporesi EM
FAU - Hakim, T S
AU  - Hakim TS
FAU - Levine, R A
AU  - Levine RA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Acidosis/*complications
MH  - Anesthesia
MH  - Animals
MH  - Blood Pressure
MH  - Carbon Dioxide/blood
MH  - Disease Models, Animal
MH  - Hemodynamics
MH  - Hydrogen-Ion Concentration
MH  - Intestinal Diseases/*etiology/pathology
MH  - Intestinal Mucosa/enzymology
MH  - *Intestine, Small/enzymology/pathology
MH  - Kinetics
MH  - Lipid Peroxidation
MH  - Male
MH  - Nitrates/blood
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Synthase/analysis/metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Nitrites/blood
MH  - Oxygen/blood
MH  - Peroxidase/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2001/09/28 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/09/28 10:00
PHST- 2001/09/28 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/09/28 10:00 [entrez]
AID - S0022-2143(01)52202-7 [pii]
AID - 10.1067/mlc.2001.118176 [doi]
PST - ppublish
SO  - J Lab Clin Med. 2001 Oct;138(4):270-6. doi: 10.1067/mlc.2001.118176.