PMID- 11577158
OWN - NLM
STAT- MEDLINE
DCOM- 20020314
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 147
IP  - Pt 10
DP  - 2001 Oct
TI  - The transcriptional activator NhaR is responsible for the osmotic induction of 
      osmC(p1), a promoter of the stress-inducible gene osmC in Escherichia coli.
PG  - 2795-2803
LID - 10.1099/00221287-147-10-2795 [doi]
AB  - Two overlapping promoters, osmC(p1) and osmC(p2), direct the transcription of the 
      osmC gene of Escherichia coli. The proximal promoter, osmC(p2), is induced upon 
      entry into stationary phase under the control of Esigma(s), the RNA polymerase 
      that uses the sigma(s) (RpoS) sigma factor. Transcription from the distal 
      promoter, osmC(p1), is independent of sigma(s). Previous analysis demonstrated 
      that the osmolarity of the growth medium modulates expression of both promoters. 
      The use of an E. coli genomic library showed that the cloned nhaR gene was able 
      to stimulate transcription of an osmC-lac reporter fusion. NhaR is a positive 
      regulator of the LysR family, previously identified as an activator of nhaA, a 
      gene encoding a Na+/H+ antiporter involved in adaptation to Na+ and alkaline pH 
      in E. coli and other enteric bacteria. NhaR was shown to activate only the 
      expression of osmC(p1) and to be necessary for the induction of this promoter by 
      LiCl, NaCl and sucrose. Therefore, activation by NhaR is responsible for the 
      osmotic induction of osmC(p1). In contrast to its action on nhaA, NhaR activation 
      of osmC(p1) is independent of H-NS. Activation of osmC(p1) by NhaR requires a 
      site located just upstream of the atypical -35 region of the promoter.
FAU - Toesca, Isabelle
AU  - Toesca I
AD  - Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS - Universite 
      Toulouse III, 118 Route de Narbonne, F-31062, Toulouse Cedex, France1.
FAU - Perard, Catherine
AU  - Perard C
AD  - Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS - Universite 
      Toulouse III, 118 Route de Narbonne, F-31062, Toulouse Cedex, France1.
FAU - Bouvier, Jean
AU  - Bouvier J
AD  - Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS - Universite 
      Toulouse III, 118 Route de Narbonne, F-31062, Toulouse Cedex, France1.
FAU - Gutierrez, Claude
AU  - Gutierrez C
AD  - Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS - Universite 
      Toulouse III, 118 Route de Narbonne, F-31062, Toulouse Cedex, France1.
FAU - Conter, Annie
AU  - Conter A
AD  - Laboratoire de Microbiologie et Genetique Moleculaire, UMR 5100 CNRS - Universite 
      Toulouse III, 118 Route de Narbonne, F-31062, Toulouse Cedex, France1.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (nhaR protein, E coli)
RN  - 0 (osmC protein, E coli)
SB  - IM
MH  - Bacterial Proteins/drug effects/genetics/*metabolism
MH  - *DNA-Binding Proteins
MH  - Escherichia coli/drug effects/genetics/*physiology
MH  - *Escherichia coli Proteins
MH  - *Gene Expression Regulation, Bacterial
MH  - Osmotic Pressure
MH  - *Promoter Regions, Genetic
MH  - Transcription Factors/genetics/*metabolism
MH  - *Transcriptional Activation
MH  - Water-Electrolyte Balance
EDAT- 2001/09/29 10:00
MHDA- 2002/03/15 10:01
CRDT- 2001/09/29 10:00
PHST- 2001/09/29 10:00 [pubmed]
PHST- 2002/03/15 10:01 [medline]
PHST- 2001/09/29 10:00 [entrez]
AID - 10.1099/00221287-147-10-2795 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2001 Oct;147(Pt 10):2795-2803. doi: 
      10.1099/00221287-147-10-2795.