PMID- 11578597
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20191210
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 914
IP  - 1-2
DP  - 2001 Sep 28
TI  - Function of SERCA mediated calcium uptake and expression of SERCA3 in cerebral 
      cortex from young and old rats.
PG  - 57-65
AB  - Previous work on peripheral sympathetic neurons indicated that a decline in 
      sarco/endoplasmic reticulum calcium ATPase (SERCA) function occurs with advancing 
      age. Therefore, an age-related decline in mechanisms controlling intracellular 
      calcium homeostasis could contribute to altered neuronal function and/or 
      degeneration. In this study we sought to extend the findings on peripheral 
      neurons and to detect possible age-related declines in SERCA function and 
      expression of SERCA3 in central neurons from cerebral cortex from young (6-month) 
      and old (20-month) rats. Functional studies compared ATP-dependent 
      45Ca(2+)-uptake into microsomes and plasma membrane vesicles (PMVs). We and found 
      no significant difference in 45Ca(2+)-uptake between microsomes or PMVs between 
      young and old animals. On the other hand expression of SERCA3 mRNA in rat 
      cerebral cortex showed a significant decline with advancing age. However, 
      comparison of SERCA3 protein content did not reveal a corresponding decline; 
      implying that SERCA mRNA turnover rates may be greater in the younger group. 
      Although the present work with rat cerebral cortex does not indicate an 
      age-related decline in SERCA function, previous work from our laboratory on 
      sympathetic nerves and by others on the hippocampus indicate such a decline. In 
      light of our previous and current studies, aging may affect calcium homeostatic 
      mechanisms in central and peripheral autonomic neurons differently.
FAU - Pottorf, W J
AU  - Pottorf WJ
AD  - Department of Pharmacology, Loma Linda University School of Medicine, Loma Linda, 
      CA 92350, USA.
FAU - De Leon, D D
AU  - De Leon DD
FAU - Hessinger, D A
AU  - Hessinger DA
FAU - Buchholz, J N
AU  - Buchholz JN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Atp2a3 protein, rat)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Calcium/*metabolism/pharmacokinetics
MH  - Calcium-Transporting ATPases/genetics/*metabolism
MH  - Cell Membrane/drug effects/enzymology
MH  - Cerebral Cortex/cytology/drug effects/*enzymology
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation, Enzymologic/physiology
MH  - Homeostasis/genetics
MH  - Intracellular Fluid/drug effects/enzymology
MH  - Male
MH  - Microsomes/drug effects/enzymology
MH  - Nerve Degeneration/*enzymology/physiopathology
MH  - Neurons/cytology/drug effects/*enzymology
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases
MH  - Subcellular Fractions/drug effects/enzymology
MH  - Thapsigargin/pharmacology
MH  - Transport Vesicles/drug effects/*enzymology
EDAT- 2001/10/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/02 10:00
PHST- 2001/10/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/02 10:00 [entrez]
AID - S0006-8993(01)02773-1 [pii]
AID - 10.1016/s0006-8993(01)02773-1 [doi]
PST - ppublish
SO  - Brain Res. 2001 Sep 28;914(1-2):57-65. doi: 10.1016/s0006-8993(01)02773-1.