PMID- 11578800
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 167
IP  - 3
DP  - 2001 Oct 30
TI  - Comparison of dose-responses of contact allergens using the guinea pig 
      maximization test and the local lymph node assay.
PG  - 207-15
AB  - The guinea pig maximization test (GPMT) has been used as a method for the 
      prediction of skin sensitizing potential for over 30 years. Besides hazard 
      identification, risk assessment of sensitizing chemicals requires the assessment 
      of potency. For the determination of potency based on lowest effective dose 
      levels, dose-response studies are required. In the standard GPMT a single 
      concentration is used for intracutaneous and topical induction and the assay 
      provides a qualitative assessment of allergenicity. This paper presents data 
      derived from quantitative evaluation of the sensitizing potency of chemicals in 
      the GPMT, based on multiple concentrations. We performed the GPMT in accordance 
      with the original procedure of Magnusson and Kligman; and included in this 
      procedure a range of intradermal and topical concentrations for induction. Three 
      allergens with different sensitizing potencies, diethylamine (DEA), tetramethyl 
      thiuram disulfide (TMTD) and zinc dimethyl dithiocarbamate (ZDMC) were tested. 
      The data obtained with this test procedure were compared to data we previously 
      obtained using the local lymph node assay (LLNA). Both the GPMT and the LLNA 
      showed dose response relationships for the three chemicals tested. For the 
      chemicals tested, both tests differed in the relative potencies based on 
      benchmark concentrations. While both tests ranked DEA as the least potent 
      allergen, the GPMT ranked ZDMC more potent than TMTD, the reverse being found in 
      the LLNA. The nature of the data provided in the LLNA makes it likely that 
      benchmarks as defined with this test are more reliable than that defined in the 
      GPMT. However, further validation with human data is necessary.
FAU - van Och, F M
AU  - van Och FM
AD  - National Institute of Public Health and the Environment (RIVM), P.O. Box 1, 3720 
      BA, Bilthoven, The Netherlands.
FAU - Vandebriel, R J
AU  - Vandebriel RJ
FAU - Prinsen, M K
AU  - Prinsen MK
FAU - De Jong, W H
AU  - De Jong WH
FAU - Slob, W
AU  - Slob W
FAU - van Loveren, H
AU  - van Loveren H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Allergens)
RN  - 0 (Diethylamines)
RN  - 0D771IS0FH (Thiram)
RN  - 73D8UA974J (Ziram)
RN  - B035PIS86W (diethylamine)
SB  - IM
EIN - Toxicology 2002 Jan 25;170(3):228-30
MH  - Allergens/*toxicity
MH  - Animals
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Diethylamines/toxicity
MH  - Dose-Response Relationship, Immunologic
MH  - Edema/chemically induced/pathology
MH  - Erythema/chemically induced/pathology
MH  - Guinea Pigs
MH  - *Local Lymph Node Assay
MH  - Male
MH  - Mice
MH  - Skin/drug effects/pathology
MH  - Thiram/toxicity
MH  - Time Factors
MH  - Ziram/toxicity
EDAT- 2001/10/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/02 10:00
PHST- 2001/10/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/02 10:00 [entrez]
AID - S0300483X01004255 [pii]
AID - 10.1016/s0300-483x(01)00425-5 [doi]
PST - ppublish
SO  - Toxicology. 2001 Oct 30;167(3):207-15. doi: 10.1016/s0300-483x(01)00425-5.