PMID- 11579460
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 3
DP  - 2001 Nov
TI  - Genome-wide analysis of sixteen chordomas by comparative genomic hybridization 
      and cytogenetics of the first human chordoma cell line, U-CH1.
PG  - 203-11
AB  - Cytogenetic information on chordomas is rudimentary and restricted to GTG-banding 
      analysis of 26 cases worldwide. In this study, we present the chromosomal 
      imbalances detected in a series of 16 chordomas (10 sacrococcyeal, five 
      sphenooccipital, and one spinal) from 13 patients using comparative genomic 
      hybridization (CGH) and fluorescence in situ hybridization (FISH). On average, 
      3.2 losses and 4.2 gains were detected per tumor. The most common DNA copy number 
      alterations were losses on chromosomal arms 3p (50%) and 1p (44%). Losses of 3p 
      were detected in five of seven primary chordomas. Therefore, the loss of 3p might 
      be an early event in chordoma genesis. The most common gains involved 7q (69%), 
      20 (50%), 5q (38%), and 12q (38%). Additionally, we raised the first human 
      chordoma cell line, U-CH1, from a recurrence of a sacral chordoma. U-CH1 and its 
      parent tumor had almost the same CGH profile. According to GTG-banding and 
      multicolor FISH, U-CH1 has the following clonal chromosomal abnormalities: 
      der(1)t(1;22), del(4), +del(5), +del(6), +7, del(9), del(10), +der(20)t(10;20), 
      +21. Thus, the novel permanent human chordoma cell line U-CH1 has 
      chordoma-typical cytogenetic aberrations. Our data suggest that tumor suppressor 
      genes or mismatch repair genes (located at 1p31 and 3p14) and oncogenes (located 
      in 7q36) might be involved in chordoma genesis.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Scheil, S
AU  - Scheil S
AD  - Institute of Pathology, University Hospitals of Ulm, Ulm, Germany. 
      stefanie.scheil@medizin.uni-ulm.de
FAU - Bruderlein, S
AU  - Bruderlein S
FAU - Liehr, T
AU  - Liehr T
FAU - Starke, H
AU  - Starke H
FAU - Herms, J
AU  - Herms J
FAU - Schulte, M
AU  - Schulte M
FAU - Moller, P
AU  - Moller P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Culture Techniques
MH  - Cell Separation
MH  - Chordoma/*genetics/*pathology
MH  - Chromosome Banding
MH  - Female
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase/genetics
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
MH  - Skull Base Neoplasms/genetics/pathology
MH  - Spinal Neoplasms/genetics/pathology
MH  - Tumor Cells, Cultured/*pathology
EDAT- 2001/10/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/02 10:00
PHST- 2001/10/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/02 10:00 [entrez]
AID - 10.1002/gcc.1184 [pii]
AID - 10.1002/gcc.1184 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Nov;32(3):203-11. doi: 10.1002/gcc.1184.