PMID- 11579465
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20191210
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 3
DP  - 2001 Nov
TI  - Novel evidence of a role for chromosome 1 pericentric heterochromatin in the 
      pathogenesis of B-cell lymphoma and multiple myeloma.
PG  - 250-64
AB  - 1q rearrangement is a remarkably frequent secondary chromosomal change in both 
      non-Hodgkin's lymphoma (NHL) and multiple myeloma (MM), where it is associated 
      with tumor progression. To gain insight into 1q rearrangement-associated disease 
      mechanisms, we used fluorescence in situ hybridization (FISH) to search for 
      recurring 1q breaks in 35 lymphoma samples (31 NHL patients and 4 
      lymphoma-derived cell lines) as well as 22 MM patients with cytogenetically 
      determined 1q abnormalities. Strikingly, dual-color FISH analysis with chromosome 
      1 centromere and 1q12-specific probes identified constitutive heterochromatin 
      band 1q12 as the single most frequent breakpoint site in both NHL and MM (39% and 
      89% of 1q breaks, respectively). These rearrangements consistently generated 
      aberrant heterochromatin/euchromatin junctions and gain of 1q12 material. A 
      further 30% of NHL 1q breaks specifically involved two other novel, closely 
      spaced sites (clusters I and II) within a 2.5 Mb region of proximal 1q21 (D1S3620 
      to D1S3623). A possible association between these sites and NHL subtype was 
      evident; the cluster I rearrangement was frequent in follicular and diffuse large 
      cell lymphoma, whereas the cluster II rearrangement was more frequently observed 
      in diffuse small-cell lymphoma (2/2 marginal zone lymphomas, 1/2 atypical chronic 
      lymphocytic leukemias, and 1 lymphoplasmacytic lymphoma in this series). 
      Candidate oncogenes bordering this interval (BCL9 and AF1Q) were not rearranged 
      in any patient except one (AF1Q). This study provides the first evidence of 
      involvement of 1q12 constitutive heterochromatin in the pathogenesis of NHL and 
      MM and indicates proximal 1q21 to be of specific pathological significance in 
      NHL.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Le Baccon, P
AU  - Le Baccon P
AD  - The Lymphoma Research Group, Institut Albert Bonniot, Universite Joseph Fourier, 
      Grenoble, France.
FAU - Leroux, D
AU  - Leroux D
FAU - Dascalescu, C
AU  - Dascalescu C
FAU - Duley, S
AU  - Duley S
FAU - Marais, D
AU  - Marais D
FAU - Esmenjaud, E
AU  - Esmenjaud E
FAU - Sotto, J J
AU  - Sotto JJ
FAU - Callanan, M
AU  - Callanan M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (BCL9 protein, human)
RN  - 0 (Blood Proteins)
RN  - 0 (Heterochromatin)
RN  - 0 (MLLT11 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blood Proteins/genetics
MH  - Chromosome Banding
MH  - Chromosome Breakage/genetics
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Female
MH  - Heterochromatin/genetics/*physiology
MH  - Humans
MH  - Karyotyping
MH  - Lymphoma, B-Cell/etiology/*genetics
MH  - Lymphoma, Non-Hodgkin/genetics
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/etiology/*genetics
MH  - Neoplasm Proteins/genetics
MH  - Proto-Oncogene Proteins
MH  - Repetitive Sequences, Nucleic Acid/genetics
MH  - Sequence Deletion/genetics
MH  - Telomere/genetics
MH  - Transcription Factors
MH  - Translocation, Genetic/genetics
EDAT- 2001/10/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/02 10:00
PHST- 2001/10/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/02 10:00 [entrez]
AID - 10.1002/gcc.1189 [pii]
AID - 10.1002/gcc.1189 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Nov;32(3):250-64. doi: 10.1002/gcc.1189.