PMID- 11579466
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 3
DP  - 2001 Nov
TI  - Detection of illegitimate rearrangement within the immunoglobulin locus on 
      14q32.3 in B-cell malignancies using end-sequenced probes.
PG  - 265-74
AB  - Translocation involving the immunoglobulin heavy chain (IGH) locus is a recurring 
      event in B-cell oncogenesis. The aim of this study was to characterize clones 
      from bacterial artificial chromosome (BAC) libraries and/or bacteriophage P1 
      artificial chromosome libraries spanning the IGH locus for detection of 
      illegitimate rearrangement within the region by fluorescence in situ 
      hybridization (FISH). In silico analysis of the IGH variable (IGHV) DNA sequence 
      (NT_001716.v1) was performed to identify BAC probes located within the IGHV 
      cluster. Clones of the constant (IGHC) cluster were found in the literature or at 
      http://www.biologia.uniba.it/rmc/. Validation, orientation, and overlap of these 
      probes were confirmed using interphase-, metaphase-, and fiber-FISH. We have 
      identified seven BAC end-sequenced probes (3087C18, 47P23, 76N15, 12F16, 101G24, 
      112H5, and 151B17) covering 612 kb of the distal IGHV cluster, which, together 
      with probes covering the IGHC cluster (11771 and 998D24), could be used in 
      interphase nuclei and metaphase chromosome analysis. A visual split of the IGHV 
      and IGHC clusters indicating a translocation was analyzed by dual-color FISH in a 
      series of 21 cell lines of different origins. Translocations were found, as 
      expected, in eight of eight myelomas, four of four lymphomas, none of five 
      leukemias, and none of four Epstein-Barr virus-transformed B-lymphoblastoid cell 
      lines. To summarize, we have established a set of IGHV and IGHC probes that can 
      be used for universal screening of illegitimate rearrangement within the IGH 
      locus in B-cell malignancies. These probes allow for routine FISH analysis to 
      detect this early central oncogenic event.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Poulsen, T S
AU  - Poulsen TS
AD  - Research Laboratory, Department of Haematology L, Herlev Hospital, University of 
      Copenhagen, Denmark. tim.poulsen@DAKO.dk
FAU - Silahtaroglu, A N
AU  - Silahtaroglu AN
FAU - Gisselo, C G
AU  - Gisselo CG
FAU - Gaarsdal, E
AU  - Gaarsdal E
FAU - Rasmussen, T
AU  - Rasmussen T
FAU - Tommerup, N
AU  - Tommerup N
FAU - Johnsen, H E
AU  - Johnsen HE
LA  - eng
SI  - GENBANK/AZ579057
SI  - GENBANK/AZ579058
SI  - GENBANK/AZ579059
SI  - GENBANK/AZ579060
SI  - GENBANK/AZ579061
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Chromosome Banding
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Human, Pair 15/genetics
MH  - Chromosomes, Human, Pair 16/genetics
MH  - DNA Probes/*genetics
MH  - *Gene Rearrangement, B-Lymphocyte, Heavy Chain
MH  - Genetic Markers/genetics
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lymphoma, B-Cell/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization/methods
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2001/10/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/02 10:00
PHST- 2001/10/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/02 10:00 [entrez]
AID - 10.1002/gcc.1193 [pii]
AID - 10.1002/gcc.1193 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Nov;32(3):265-74. doi: 10.1002/gcc.1193.