PMID- 11581383
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 75
IP  - 21
DP  - 2001 Nov
TI  - The R3 region, one of three major repetitive regions of human herpesvirus 6, is a 
      strong enhancer of immediate-early gene U95.
PG  - 10149-60
AB  - An immediate-early (IE) gene of human herpesvirus 6 (HHV-6), U95, has similarity 
      at the amino acid level to the murine cytomegalovirus (MCMV) IE2 gene and is 
      related to the human cytomegalovirus (HCMV) US22 gene family. Sequence analyses 
      of U95 cDNA clones revealed that the transcription start site was located about 
      1.6 kbp upstream of the putative initiating ATG and that the transcript consisted 
      of two exons. A single intron extended from nucleotides 142589 to 144229, which 
      contained ORF U94. A protein with a molecular mass of about 120 kDa was 
      translated from this cDNA clone in an in vitro transcription-translation assay. 
      The transcription start site was found to be 220 bp downstream of the R3 region 
      by primer extension analysis. HHV-6 has three repetitive elements, R1, R2, and 
      R3, in or near the IE-A locus. R3 is composed of 24 copies of a 104- to 107-bp 
      sequence element, which contains multiple putative binding sites for cellular 
      transcription factors such as AP2 and NF-kappaB, and its biological significance 
      has yet to be elucidated. The region between -710 and +46 relative to the 
      transcription start site of U95 was analyzed in this study. Deletion from -710 to 
      -396, corresponding to three copies of an R3 unit, decreased the promoter 
      activity by 15-fold, and coexpression of IkappaBalpha(S32A/S36A) repressed it to 
      almost the same level. Electrophoretic mobility shift assays showed that 
      NF-kappaB family members p50 and c-Rel bound to NF-kappaB sites derived from the 
      R3 region. These results demonstrate that R3 strongly enhances the U95 promoter 
      activity and that NF-kappaB and binding sites for NF-kappaB in the R3 region play 
      an important role in its activation. Because U95 promoter activity correlated 
      with the number of R3 units, which each contained an NF-kappaB site, the 
      repetitive organization of R3 is important for regulating U95 transcription.
FAU - Takemoto, M
AU  - Takemoto M
AD  - Department of Microbiology, Osaka University Medical School C1, Suita, Osaka 
      565-0871, Japan.
FAU - Shimamoto, T
AU  - Shimamoto T
FAU - Isegawa, Y
AU  - Isegawa Y
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Base Sequence
MH  - DNA, Viral/*chemistry
MH  - *Enhancer Elements, Genetic
MH  - Exons
MH  - *Genes, Immediate-Early
MH  - *Genes, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Molecular Sequence Data
MH  - NF-kappa B/physiology
MH  - Promoter Regions, Genetic
MH  - RNA Splicing
MH  - RNA, Messenger/analysis
MH  - *Repetitive Sequences, Nucleic Acid
PMC - PMC114589
EDAT- 2001/10/03 10:00
MHDA- 2001/10/26 10:01
PMCR- 2001/11/01
CRDT- 2001/10/03 10:00
PHST- 2001/10/03 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/10/03 10:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - 0539 [pii]
AID - 10.1128/JVI.75.21.10149-10160.2001 [doi]
PST - ppublish
SO  - J Virol. 2001 Nov;75(21):10149-60. doi: 10.1128/JVI.75.21.10149-10160.2001.