PMID- 11585299
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20190826
IS  - 0340-6997 (Print)
IS  - 0340-6997 (Linking)
VI  - 28
IP  - 9
DP  - 2001 Sep
TI  - Imaging macrophages and the apoptosis of granulocytes in a rodent model of 
      subacute and chronic abscesses with radiolabeled monocyte chemotactic peptide-1 
      and annexin V.
PG  - 1384-93
AB  - Monocytes/macrophages (Mphis), the predominant cell types in subacute and chronic 
      inflammation, are attracted to and activated by monocyte chemotactic peptide-1 
      (MCP-1). Mphis promote the resolution of inflammation through the induction of 
      apoptosis and phagocytosis of senescent (spent) and bystander (superfluous) 
      granulocytes. We wished to determine whether MCP-1, which selectively binds to 
      Mphis, could be used to image subacute and chronic inflammation. We also sought 
      to image granulocyte apoptosis within these lesions with technetium-99m labeled 
      annexin V, a marker of apoptotic cells. Sterile inflammation was induced in 45 
      12-week-old male Sprague-Dawley rats by deep intramuscular injection of 
      turpentine into the right thigh. Groups of four to six animals were then imaged 1 
      h after tail vein injection of 37-148 MBq (1-4 mCi) of 99mTc-labeled MCP-1 or 
      annexin V 1-14 days after turpentine treatment. Image analysis showed 
      significantly greater activity of both MCP-1 and annexin V in inflamed thighs 
      than in control thighs (165%-290% and 188%-313%, respectively; P<0.01) on days 
      1-5 after turpentine injection. Dual autoradiography in animals co-injected with 
      iodine-125 labeled bovine serum albumin on days 1 and 4 showed specific location 
      of MCP-1 to infiltrating Mphis while annexin V localized to focal zones of 
      apoptosis within granulocytic infiltrates adjacent to abscess cavities. 
      Scintillation well counting on day 5 demonstrated significantly higher (P<0.005) 
      ratios of abscess to control thigh specific activities for MCP-1 (5.83+/-2.17) 
      and annexin V (9.24 +/- 2.8) as compared to 125I-labeled bovine serum albumin 
      (3.11 +/- 0.65). No significant increases in uptake were noted at imaging or ex 
      vivo analyses on days 13 and 14, when lesions were predominately fibrotic. It is 
      concluded that 99mTc-labeled MCP-1 and 99mTc-labeled annexin V both localize in 
      zones of subacute inflammation, reflecting the density of Mphis and the incidence 
      of apoptotic granulocytes, respectively. These agents may be useful in the 
      characterization of subacute inflammation.
FAU - Blankenberg, F G
AU  - Blankenberg FG
AD  - Division of Pediatric Radiology, Lucile Salter Packard Children's Hospital, 
      Stanford, Calif, USA. blankenb@leland.stanford.edu
FAU - Tait, J F
AU  - Tait JF
FAU - Blankenberg, T A
AU  - Blankenberg TA
FAU - Post, A M
AU  - Post AM
FAU - Strauss, H W
AU  - Strauss HW
LA  - eng
GR  - HL-61717/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Eur J Nucl Med
JT  - European journal of nuclear medicine
JID - 7606882
RN  - 0 (Annexin A5)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Serum Albumin, Radio-Iodinated)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7440-26-8 (Technetium)
SB  - IM
MH  - Abscess/*diagnostic imaging/pathology
MH  - Animals
MH  - *Annexin A5
MH  - *Apoptosis
MH  - Autoradiography
MH  - *Chemokine CCL2/pharmacokinetics
MH  - Chronic Disease
MH  - Granulocytes/*diagnostic imaging
MH  - Hindlimb
MH  - Macrophages/*diagnostic imaging
MH  - Male
MH  - Monocytes/diagnostic imaging
MH  - Radionuclide Imaging
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recombinant Proteins
MH  - Scintillation Counting
MH  - Serum Albumin, Bovine
MH  - Serum Albumin, Radio-Iodinated
MH  - *Technetium/pharmacokinetics
MH  - Tissue Distribution
EDAT- 2001/10/05 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/10/05 10:00
PHST- 2001/10/05 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/10/05 10:00 [entrez]
AID - 10.1007/s002590100572 [doi]
PST - ppublish
SO  - Eur J Nucl Med. 2001 Sep;28(9):1384-93. doi: 10.1007/s002590100572.