PMID- 11585581
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 310
IP  - 2-3
DP  - 2001 Sep 14
TI  - Riluzole stimulates nerve growth factor, brain-derived neurotrophic factor and 
      glial cell line-derived neurotrophic factor synthesis in cultured mouse 
      astrocytes.
PG  - 117-20
AB  - Riluzole is an antiexcitotoxic agent used for the treatment of amyotrophic 
      lateral sclerosis, and reported to have neuroprotective effects in animal models 
      of Parkinson's disease, Huntington's disease and brain ischemia. We investigated 
      the effects of riluzole on synthesis of nerve growth factor (NGF), brain-derived 
      neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) 
      in cultured mouse astrocytes. The protein and mRNA levels were measured by 
      enzyme-linked immunosorbent assay and semiquantitative reverse 
      transcription-polymerase chain reaction, respectively. Treatment with riluzole at 
      100 microg/ml (426 microM) for 24 h increased the contents of NGF, BDNF, and GDNF 
      in the culture medium 109-fold, 2.0-fold and 3.1-fold over the control, 
      respectively. The drug-induced relative mRNA levels of NGF, BDNF, and GDNF were 
      7.3-fold at 2 h, 2.1-fold at 4 h, and 1.9-fold at 2 h, respectively. These 
      results indicate that riluzole stimulates synthesis of NGF, BDNF and GDNF in 
      cultured astrocytes. Riluzole might exert neuroprotective effects, at least in 
      part, via stimulation of neurotrophic factors.
FAU - Mizuta, I
AU  - Mizuta I
AD  - Clinical Research Center, Utano National Hospital, Narutaki, Ukyo-ku, Kyoto 
      616-8255, Japan.
FAU - Ohta, M
AU  - Ohta M
FAU - Ohta, K
AU  - Ohta K
FAU - Nishimura, M
AU  - Nishimura M
FAU - Mizuta, E
AU  - Mizuta E
FAU - Kuno, S
AU  - Kuno S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, mouse)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 7LJ087RS6F (Riluzole)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Astrocytes/cytology/*drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/biosynthesis
MH  - Cells, Cultured
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nerve Growth Factor/biosynthesis
MH  - Nerve Growth Factors/*biosynthesis
MH  - Nerve Tissue Proteins/biosynthesis
MH  - Neuroprotective Agents/*pharmacology
MH  - Riluzole/*pharmacology
EDAT- 2001/10/05 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/05 10:00
PHST- 2001/10/05 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/05 10:00 [entrez]
AID - S0304394001020985 [pii]
AID - 10.1016/s0304-3940(01)02098-5 [doi]
PST - ppublish
SO  - Neurosci Lett. 2001 Sep 14;310(2-3):117-20. doi: 10.1016/s0304-3940(01)02098-5.