PMID- 11586495
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20131121
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 50
IP  - 10
DP  - 2001 Oct
TI  - Glucose uptake and glycolytic flux in adipose tissue from rats adapted to a 
      high-protein, carbohydrate-free diet.
PG  - 1208-12
AB  - Rates of glucose uptake by epididymal and retroperitoneal adipose tissue in vivo, 
      as well as rates of hexose uptake and glycolytic flux in isolated adipocytes, 
      were determined in rats adapted to a high-protein, carbohydrate-free (HP) diet 
      and in control rats fed a balanced (N) diet. Adaptation to the HP diet induced a 
      significant reduction in rates of glucose uptake, estimated with 
      2-deoxy-[1-(3)H]-glucose, both by adipose tissue (epididymal and retroperitoneal) 
      in vivo and by isolated adipocytes. Twelve hours after replacement of the HP diet 
      with the balanced diet, rates of adipose tissue uptake in vivo in HP-adapted rats 
      returned to levels that did not differ significantly from those in N-fed rats. 
      The rate of flux in the glycolytic pathway, estimated with (3)H[5]-glucose, was 
      also significantly reduced in adipocytes from HP-fed rats. In agreement with the 
      above findings, the activities of hexokinase (HK), phosphofructo-1-kinase 
      (PFK-1), and pyruvate kinase (PK) were markedly reduced in adipose tissue from 
      HP-adapted rats. The activity of pyruvate kinase was partially reverted by diet 
      replacement for 12 hours. The low-plasma insulin and high-glucagon levels in 
      HP-fed rats may have played an important role in the reduction of adipose tissue 
      glucose utilization in these animals.
CI  - Copyright 2001 by W.B. Saunders Company
FAU - Brito, S R
AU  - Brito SR
AD  - Department of Biochemistry, School of Medicine, University of Sao Paulo, Sao 
      Paulo, Brazil.
FAU - Moura, M A
AU  - Moura MA
FAU - Kawashita, N H
AU  - Kawashita NH
FAU - Brito, M N
AU  - Brito MN
FAU - Kettelhut, I C
AU  - Kettelhut IC
FAU - Migliorini, R H
AU  - Migliorini RH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Carbohydrates)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Insulin)
RN  - 0 (Proteins)
RN  - 10028-17-8 (Tritium)
RN  - 9007-92-5 (Glucagon)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - EC 2.7.1.56 (1-phosphofructokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipose Tissue/cytology/*metabolism
MH  - Animals
MH  - Carbohydrates/*administration & dosage
MH  - Carbon Radioisotopes
MH  - Epididymis
MH  - Glucagon/blood
MH  - Glucose/*metabolism
MH  - Glycolysis
MH  - Hexokinase/metabolism
MH  - Insulin/blood
MH  - Male
MH  - Phosphofructokinase-1/metabolism
MH  - Proteins/*administration & dosage
MH  - Pyruvate Kinase/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tritium
EDAT- 2001/10/05 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/10/05 10:00
PHST- 2001/10/05 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/10/05 10:00 [entrez]
AID - S0026-0495(01)81579-1 [pii]
AID - 10.1053/meta.2001.25645 [doi]
PST - ppublish
SO  - Metabolism. 2001 Oct;50(10):1208-12. doi: 10.1053/meta.2001.25645.