PMID- 11587222
OWN - NLM
STAT- MEDLINE
DCOM- 20020222
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 15
IP  - 10
DP  - 2001 Oct
TI  - Chromosomal changes pattern and gene amplification in T cell non-Hodgkin's 
      lymphomas.
PG  - 1627-32
AB  - T cell non-Hodgkin's lymphomas are a heterogeneous group of lymphomas with poor 
      prognosis, and whose genetic alterations are not well understood. Comparative 
      genomic hybridization (CGH) is a technique that allows the identification of DNA 
      imbalances without cytogenetic studies. We have studied 37 samples from 29 T cell 
      non-Hodgkin's lymphomas (25 peripheral and four lymphoblastic lymphomas) by CGH 
      in order to detect DNA sequence copy number changes of putative importance in the 
      biology and prognosis of these neoplasms. We detected abnormal CGH profiles in 
      16/27 (59%) of samples at diagnosis, a ratio that increased to 66% (23/37) when 
      we included the relapsed samples. The most common recurrent changes were gains 
      related to the X chromosome, either the whole chromosome or partially the Xq26-27 
      bands (19%). Other recurrent changes included gains of bands 9q34, gains of 
      chromosomes 17, 19, and 20, and complete or partial deletions of chromosome 13 
      (10%). Cancer-related genes located at Xq26-28 region were analyzed by Southern 
      blot and fluorescence in situ hybridization (FISH). Low level amplification of 
      some of these genes was detected by this technique confirming the results 
      obtained by CGH in this region. The detection of abnormal CGH profiles in these T 
      cell lymphomas could have clinical implications. Patients with abnormal CGH 
      profiles showed significant associations with advanced stage of disease, 
      overexpression of P53, and higher proliferative index.
FAU - Renedo, M
AU  - Renedo M
AD  - Department of Human Genetics, Centro Nacional de Investigaciones Oncologicas, 
      Madrid, Spain.
FAU - Martinez-Delgado, B
AU  - Martinez-Delgado B
FAU - Arranz, E
AU  - Arranz E
FAU - Garcia, M
AU  - Garcia M
FAU - Urioste, M
AU  - Urioste M
FAU - Martinez-Ramirez, A
AU  - Martinez-Ramirez A
FAU - Rivas, C
AU  - Rivas C
FAU - Cigudosa, J C
AU  - Cigudosa JC
FAU - Benitez, I
AU  - Benitez I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Blotting, Southern
MH  - Chromosome Aberrations/*genetics
MH  - *Chromosome Disorders
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Non-Hodgkin/diagnosis/genetics
MH  - Lymphoma, T-Cell/diagnosis/*genetics
MH  - Male
MH  - Nucleic Acid Hybridization/methods
EDAT- 2001/10/06 10:00
MHDA- 2002/02/23 10:01
CRDT- 2001/10/06 10:00
PHST- 2001/10/06 10:00 [pubmed]
PHST- 2002/02/23 10:01 [medline]
PHST- 2001/10/06 10:00 [entrez]
AID - 10.1038/sj.leu.2402248 [doi]
PST - ppublish
SO  - Leukemia. 2001 Oct;15(10):1627-32. doi: 10.1038/sj.leu.2402248.