PMID- 11587512
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20190822
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 47
IP  - 3
DP  - 2001 Oct
TI  - Visualization of the terminal structure of rice chromosomes 6 and 12 with 
      multicolor FISH to chromosomes and extended DNA fibers.
PG  - 413-21
AB  - High-resolution fluorescence in situ hybridization (FISH) on interphase and 
      pachytene nuclei, and extended DNA fibers enabled microscopic distinction of DNA 
      sequences less than a few thousands of base pairs apart. We applied this 
      technique to reveal the molecular organization of telomere ends in japonica rice 
      (Oryza sativa ssp. japonica), which consist of the Arabidopsis type TTTAGGG 
      heptameric repeats and the rice specific subtelomeric tandem repeat sequence A 
      (TrsA). Southern hybridizations of DNA digested with Bal31 and EcoRI, and FISH on 
      chromosomes and extended DNA fibers demonstrated that (1) all chromosome ends 
      possess the telomere tandem repeat measuring 3-4 kb; (2) the subtelomeric TrsA 
      occurs only at the ends of the long arms of chromosomes 6 and 12, and measure 6 
      and 10 kb, which corresponds to 231 and 682 copies for these sites, respectively; 
      (3) the telomere and TrsA repeats are separated by at most a few thousands of 
      intervening nucleotide sequences. The molecular organization for a general 
      telomere organization in plant chromosomes is discussed.
FAU - Ohmido, N
AU  - Ohmido N
AD  - National Agricultural Research Center, Joetsu, Japan.
FAU - Kijima, K
AU  - Kijima K
FAU - Ashikawa, I
AU  - Ashikawa I
FAU - de Jong, J H
AU  - de Jong JH
FAU - Fukui, K
AU  - Fukui K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Chromosome Painting
MH  - Chromosomes/*genetics
MH  - DNA, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Oryza/*genetics
MH  - Repetitive Sequences, Nucleic Acid
MH  - Telomere/genetics
EDAT- 2001/10/06 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/10/06 10:00
PHST- 2001/10/06 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/10/06 10:00 [entrez]
AID - 10.1023/a:1011632111845 [doi]
PST - ppublish
SO  - Plant Mol Biol. 2001 Oct;47(3):413-21. doi: 10.1023/a:1011632111845.