PMID- 11587731
OWN - NLM
STAT- MEDLINE
DCOM- 20011217
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 82
IP  - 3-4
DP  - 2001 Oct
TI  - Isolation, characterisation and expression of mRNAs encoding the ovine CC 
      chemokines, monocyte chemoattractant protein (MCP)-1alpha and -2.
PG  - 153-64
AB  - CC chemokines are important mediators of immune responses, orchestrating the 
      differential recruitment of various leukocyte populations. Despite the large 
      number of known CC chemokines in other species, no cDNA encoding ovine CC 
      chemokines have been isolated. A homology cloning strategy was utilised to 
      isolate the cDNA of ovine CC chemokines. Full-length monocyte chemoattractant 
      protein (MCP)-1alpha and -2 cDNA have been isolated. The predicted ovine 
      MCP-1alpha amino acid sequence shares 87 and 75% identity with bovine MCP-1alpha 
      and porcine MCP-1, respectively. The predicted ovine MCP-2 amino acid sequence 
      shares 92 and 85% identity with bovine and porcine MCP-2, respectively. Northern 
      blot analysis of MCP-1alpha revealed that it is strongly expressed in cells 
      isolated from mammary lavage fluid (MAL) of ewes given intramammary infusions of 
      Haemonchus contortus. Weak signals were detected in mammary and abomasal tissue. 
      Southern blot analysis of reverse transcriptase-polymerase chain reaction 
      (RT-PCR) products indicates that MCP-1alpha mRNA levels increase in abomasum 
      after challenge with H. contortus. MCP-1alpha mRNA levels were also increased in 
      bronchoalveolar lavage (BAL) cells and lung tissue after house dust mite extract 
      (HDME) challenge. Similarly, MCP-2 mRNA was detected by Northern blot analysis at 
      high levels in MAL cells after H. contortus intramammary infusion, and increased 
      in BAL cells and lung tissue in HDME-challenged sheep. MCP-2 mRNA was not 
      detected by Northern blots in whole mammary or abomasal tissue, but Southern blot 
      analysis of RT-PCR products also indicates that MCP-2 mRNA increases in abomasal 
      tissue after challenge with H. contortus. Hence, two ovine CC chemokine mRNA have 
      been isolated that are up-regulated in response to parasite infection and 
      allergen challenge. Ultimately the isolation of these and other ovine CC 
      chemokines will help elucidate a wide variety of immune responses in sheep.
FAU - Dunphy, J
AU  - Dunphy J
AD  - The Centre for Animal Biotechnology, School of Veterinary Science, The University 
      of Melbourne, Victoria 3010, Australia.
FAU - Horvath, A
AU  - Horvath A
FAU - Barcham, G
AU  - Barcham G
FAU - Balic, A
AU  - Balic A
FAU - Bischof, R
AU  - Bischof R
FAU - Meeusen, E
AU  - Meeusen E
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Dust)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern/veterinary
MH  - Blotting, Southern/veterinary
MH  - Chemokine CCL2/analysis/*biosynthesis/genetics
MH  - Chemokine CCL8
MH  - DNA/chemistry
MH  - Dust
MH  - Female
MH  - Gene Expression Regulation
MH  - Haemonchiasis/immunology/*veterinary
MH  - Haemonchus/growth & development
MH  - Lung/immunology/parasitology
MH  - Male
MH  - Mammary Glands, Animal/immunology/parasitology
MH  - Molecular Sequence Data
MH  - Monocyte Chemoattractant Proteins/analysis/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis/genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sequence Homology, Amino Acid
MH  - Sheep
MH  - Sheep Diseases/immunology/*metabolism/parasitology
EDAT- 2001/10/06 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/06 10:00
PHST- 2001/10/06 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/06 10:00 [entrez]
AID - S0165242701003567 [pii]
AID - 10.1016/s0165-2427(01)00356-7 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2001 Oct;82(3-4):153-64. doi: 
      10.1016/s0165-2427(01)00356-7.