PMID- 11588046
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 98
IP  - 8
DP  - 2001 Oct 15
TI  - HIV gp120 receptors on human dendritic cells.
PG  - 2482-8
AB  - Dendritic cells (DCs) are important targets for human immunodeficiency virus 
      (HIV) because of their roles during transmission and also maintenance of immune 
      competence. Furthermore, DCs are a key cell in the development of HIV vaccines. 
      In both these settings the mechanism of binding of the HIV envelope protein gp120 
      to DCs is of importance. Recently a single C-type lectin receptor (CLR), DC-SIGN, 
      has been reported to be the predominant receptor on monocyte-derived DCs (MDDCs) 
      rather than CD4. In this study a novel biotinylated gp120 assay was used to 
      determine whether CLR or CD4 were predominant receptors on MDDCs and ex vivo 
      blood DCs. CLR bound more than 80% of gp120 on MDDCs, with residual binding 
      attributable to CD4, reconfirming that CLRs were the major receptors for gp120 on 
      MDDCs. However, in contrast to recent reports, gp120 binding to at least 3 CLRs 
      was observed: DC-SIGN, mannose receptor, and unidentified trypsin resistant 
      CLR(s). In marked contrast, freshly isolated and cultured CD11c(+ve) and 
      CD11c(-ve) blood DCs only bound gp120 via CD4. In view of these marked 
      differences between MDDCs and blood DCs, HIV capture by DCs and transfer 
      mechanisms to T cells as well as potential antigenic processing pathways will 
      need to be determined for each DC phenotype.
FAU - Turville, S G
AU  - Turville SG
AD  - Center for Virus Research, Westmead Millennium Institute, Sydney, Australia.
FAU - Arthos, J
AU  - Arthos J
FAU - Donald, K M
AU  - Donald KM
FAU - Lynch, G
AU  - Lynch G
FAU - Naif, H
AU  - Naif H
FAU - Clark, G
AU  - Clark G
FAU - Hart, D
AU  - Hart D
FAU - Cunningham, A L
AU  - Cunningham AL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (AIDS Vaccines)
RN  - 0 (CD4 Antigens)
RN  - 0 (COLEC12 protein, human)
RN  - 0 (Collectins)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Receptors, HIV)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Scavenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - AIDS Vaccines
MH  - Binding Sites
MH  - Biological Transport
MH  - CD4 Antigens/physiology
MH  - *Collectins
MH  - Dendritic Cells/*virology
MH  - HIV Envelope Protein gp120/*blood/metabolism
MH  - Humans
MH  - Kinetics
MH  - Monocytes/physiology/virology
MH  - Receptors, HIV/*physiology
MH  - Receptors, Immunologic/*physiology
MH  - Receptors, Scavenger
MH  - Recombinant Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trypsin/metabolism
MH  - Vaccines, Synthetic
EDAT- 2001/10/06 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/06 10:00
PHST- 2001/10/06 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/06 10:00 [entrez]
AID - S0006-4971(20)56939-5 [pii]
AID - 10.1182/blood.v98.8.2482 [doi]
PST - ppublish
SO  - Blood. 2001 Oct 15;98(8):2482-8. doi: 10.1182/blood.v98.8.2482.