PMID- 11590178
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20220408
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 51
DP  - 2001 Dec 21
TI  - Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that 
      promotes hepatocyte growth factor/scatter factor mitogenic activity.
PG  - 48341-9
AB  - Proteoglycans that modulate the activities of growth factors, chemokines, and 
      coagulation factors regulate in turn the vascular endothelium with respect to 
      processes such as inflammation, hemostasis, and angiogenesis. Endothelial 
      cell-specific molecule-1 is mainly expressed by endothelial cells and regulated 
      by pro-inflammatory cytokines (Lassalle, P., Molet, S., Janin, A., Heyden, J. V., 
      Tavernier, J., Fiers, W., Devos, R., and Tonnel, A. B. (1996) J. Biol. Chem. 271, 
      20458-20464). We demonstrate that this molecule is secreted as a soluble dermatan 
      sulfate (DS) proteoglycan. This proteoglycan represents the major form either 
      secreted by cell lines or circulating in the human bloodstream. Because this 
      proteoglycan is specifically secreted by endothelial cells, we propose to name it 
      endocan. The glycosaminoglycan component of endocan consists of a single DS chain 
      covalently attached to serine 137. Endocan dose-dependently increased the 
      hepatocyte growth factor/scatter factor (HGF/SF)-mediated proliferation of human 
      embryonic kidney cells, whereas the nonglycanated form of endocan did not. 
      Moreover, DS chains purified from endocan mimicked the endocan-mediated increase 
      of cell proliferation in the presence of HGF/SF. Overall, our results demonstrate 
      that endocan is a novel soluble dermatan sulfate proteoglycan produced by 
      endothelial cells. Endocan regulates HGF/SF-mediated mitogenic activity and may 
      support the function of HGF/SF not only in embryogenesis and tissue repair after 
      injury but also in tumor progression.
FAU - Bechard, D
AU  - Bechard D
AD  - INSERM U416, Institut Pasteur de Lille, 1 Rue du Dr. A Calmette, 59019 Lille, 
      France.
FAU - Gentina, T
AU  - Gentina T
FAU - Delehedde, M
AU  - Delehedde M
FAU - Scherpereel, A
AU  - Scherpereel A
FAU - Lyon, M
AU  - Lyon M
FAU - Aumercier, M
AU  - Aumercier M
FAU - Vazeux, R
AU  - Vazeux R
FAU - Richet, C
AU  - Richet C
FAU - Degand, P
AU  - Degand P
FAU - Jude, B
AU  - Jude B
FAU - Janin, A
AU  - Janin A
FAU - Fernig, D G
AU  - Fernig DG
FAU - Tonnel, A B
AU  - Tonnel AB
FAU - Lassalle, P
AU  - Lassalle P
LA  - eng
PT  - Journal Article
DEP - 20011005
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ESM1 protein, human)
RN  - 0 (Mitogens)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteoglycans)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 4.2.2.- (Chondroitinases and Chondroitin Lyases)
RN  - EC 4.2.2.- (Polysaccharide-Lyases)
RN  - EC 4.2.2.8 (heparitinsulfate lyase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blood Coagulation/physiology
MH  - CHO Cells
MH  - Cell Line
MH  - Chondroitinases and Chondroitin Lyases/metabolism
MH  - Chromatography, Gel
MH  - Cricetinae
MH  - Glycosylation
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Mitogens/*physiology
MH  - Molecular Weight
MH  - *Neoplasm Proteins
MH  - Polysaccharide-Lyases/metabolism
MH  - Proteoglycans/chemistry/*physiology
EDAT- 2001/10/09 10:00
MHDA- 2002/02/01 10:01
CRDT- 2001/10/09 10:00
PHST- 2001/10/09 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2001/10/09 10:00 [entrez]
AID - S0021-9258(19)40355-4 [pii]
AID - 10.1074/jbc.M108395200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Dec 21;276(51):48341-9. doi: 10.1074/jbc.M108395200. Epub 2001 
      Oct 5.