PMID- 11590385
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190723
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 108
IP  - 4
DP  - 2001 Oct
TI  - Functional expression of chemokine receptor 2 by normal human eosinophils.
PG  - 581-7
AB  - BACKGROUND: Within the granulocytes, the CC chemokines preferentially activate 
      basophils and eosinophils on binding to chemokine receptors (CCRs). In vivo 
      administration of neutralizing anti-monocyte chemoattractant protein 1 (MCP-1) 
      antibodies can block accumulation of eosinophils in the lungs of 
      antigen-challenged animals. OBJECTIVE: We studied a panel of chemokines for 
      chemotactic activity in normal human eosinophils from healthy donors with a 
      special focus on MCP-1, identified the respective receptor required for the 
      biological response of eosinophils, and investigated mediators used for signal 
      transduction. METHODS: Cells were enriched by magnetic cell sorting. Receptor 
      expression in eosinophils was shown by RT-PCR and fluorescence-activated cell 
      sorting. The biological response was tested in chemotaxis and calcium 
      mobilization assays. RESULTS: Eosinophils have detectable mRNA for CCR2, and the 
      receptor protein is expressed on cell surfaces. MCP-1 induces chemotaxis and 
      calcium mobilization in eosinophils. The chemotactic activity of MCP-1 revealed a 
      double-peaked dose-response curve; one of the peaks is abolished by addition of a 
      blocking antibody to CCR2, but it is insensitive to blocking of CCR1 or CCR3. 
      Specific enzyme inhibitors ruled out signaling characteristics of CCR2 in 
      eosinophils. CONCLUSION: Normal human eosinophils express functional CCR2 on cell 
      surfaces.
FAU - Dunzendorfer, S
AU  - Dunzendorfer S
AD  - Division of General Internal Medicine, Department of Internal Medicine, 
      University of Innsbruck, Austria.
FAU - Kaneider, N C
AU  - Kaneider NC
FAU - Kaser, A
AU  - Kaser A
FAU - Woell, E
AU  - Woell E
FAU - Frade, J M
AU  - Frade JM
FAU - Mellado, M
AU  - Mellado M
FAU - Martinez-Alonso, C
AU  - Martinez-Alonso C
FAU - Wiedermann, C J
AU  - Wiedermann CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Calcium Signaling
MH  - Cell Separation
MH  - Chemokine CCL2/*pharmacology
MH  - Chemotaxis, Leukocyte
MH  - Dose-Response Relationship, Drug
MH  - Eosinophils/cytology/*immunology
MH  - Humans
MH  - Monocytes/immunology
MH  - RNA, Messenger/isolation & purification
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/genetics/isolation & purification/*metabolism
EDAT- 2001/10/09 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/09 10:00
PHST- 2001/10/09 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/09 10:00 [entrez]
AID - S0091674901980844 [pii]
AID - 10.1067/mai.2001.118518 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2001 Oct;108(4):581-7. doi: 10.1067/mai.2001.118518.