PMID- 11590619
OWN - NLM
STAT- MEDLINE
DCOM- 20020213
LR  - 20191210
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 45
IP  - 2
DP  - 2001 Oct 1
TI  - Improved procedure for fluorescence in situ hybridization on tissue microarrays.
PG  - 83-6
AB  - BACKGROUND: The recently developed tissue microarray (TMA) technology allows the 
      arrangement of up to a thousand tissue specimens on a single microscope slide. 
      This technology enables researchers to perform gene copy number studies on very 
      large series of archival formalin-fixed tissues using fluorescence in situ 
      hybridization (FISH). However, the hybridization properties of individual 
      archival specimens can vary considerably. Therefore a highly optimized protocol 
      is needed to fulfill the task of producing evaluable hybridization signals 
      simultaneously in hundreds of specimens in a TMA. METHODS: The performance of two 
      different FISH protocols, the standard protocol for paraffin embedded tissues and 
      our new optimized protocol, was tested on TMAs using probes for the HER-2 and 
      ZNF217 genes as well as the chromosome 17 centromere. RESULTS: The new protocol 
      resulted in greatly increased signal intensity and an almost 30% increase in the 
      number of tissue samples with evaluable hybridization signals. CONCLUSIONS: Our 
      improved protocol for FISH on TMAs provides standardized hybridization conditions 
      leading to high-quality hybridization signals in the majority of specimens. The 
      increases in the signal intensity and the number of evaluable samples are 
      extremely important for the successful analyses of TMAs by FISH and will allow 
      the utilization of the TMA technology in its full potential.
FAU - Andersen, C L
AU  - Andersen CL
AD  - Cancer Genetics Branch, National Human Genome Research Institute, National 
      Institutes of Health, Bethesda, Maryland 20892-4465, USA.
FAU - Hostetter, G
AU  - Hostetter G
FAU - Grigoryan, A
AU  - Grigoryan A
FAU - Sauter, G
AU  - Sauter G
FAU - Kallioniemi, A
AU  - Kallioniemi A
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Autoantigens)
RN  - 0 (Centromere Protein A)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Trans-Activators)
RN  - 0 (ZNF217 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - *Autoantigens
MH  - Breast/pathology
MH  - Breast Neoplasms/*genetics/pathology
MH  - Centromere Protein A
MH  - Chromosomal Proteins, Non-Histone/genetics
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Paraffin Embedding
MH  - Receptor, ErbB-2/genetics
MH  - Trans-Activators/genetics
EDAT- 2001/10/09 10:00
MHDA- 2002/02/14 10:01
CRDT- 2001/10/09 10:00
PHST- 2001/10/09 10:00 [pubmed]
PHST- 2002/02/14 10:01 [medline]
PHST- 2001/10/09 10:00 [entrez]
AID - 10.1002/1097-0320(20011001)45:2<83::AID-CYTO1149>3.0.CO;2-P [pii]
AID - 10.1002/1097-0320(20011001)45:2<83::aid-cyto1149>3.0.co;2-p [doi]
PST - ppublish
SO  - Cytometry. 2001 Oct 1;45(2):83-6. doi: 
      10.1002/1097-0320(20011001)45:2<83::aid-cyto1149>3.0.co;2-p.