PMID- 11591377
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 506
IP  - 2
DP  - 2001 Oct 5
TI  - The generation and characterisation of antagonist RNA aptamers to MCP-1.
PG  - 85-90
AB  - Monocyte chemoattractant protein-1 (MCP-1) has been implicated as a powerful 
      pro-inflammatory mediator and may represent a potentially important, therapeutic 
      opportunity for treatment of inflammatory disease and atherosclerosis. To further 
      investigate the role of MCP-1 in inflammatory disorders we have isolated a series 
      of RNA aptamers that bind specifically to mouse MCP-1. The highest affinity 
      aptamers, designated ADR7 and ADR22, have been functionally characterised in 
      vitro and in cell based assays. ADR7 and ADR22 have an affinity of 180 pM and 370 
      pM respectively for mouse MCP-1, they can antagonise MCP-1 binding to heparin and 
      specifically antagonise MCP-1 induced chemotaxis in a cell based assay. An 
      interesting feature of ADR22 but not ADR7 is that it is capable of antagonising 
      the function of human MCP-1, demonstrating the high level of specificity of these 
      aptamers and that the aptamers recognise MCP-1 in different ways. The aptamers 
      may be used as a tool to further investigate the role of MCP-1 in inflammatory 
      disorders and may also have a role as a therapeutic agent.
FAU - Rhodes, A
AU  - Rhodes A
AD  - Molecular Discovery Department, Glaxo Wellcome Research and Development, 
      Stevenage, Herts SG1 2NY, UK. adr7003@gsk.com
FAU - Smithers, N
AU  - Smithers N
FAU - Chapman, T
AU  - Chapman T
FAU - Parsons, S
AU  - Parsons S
FAU - Rees, S
AU  - Rees S
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Anticoagulants)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Ligands)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 63231-63-0 (RNA)
RN  - 9005-49-6 (Heparin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Anticoagulants/metabolism
MH  - CHO Cells
MH  - Calcium/metabolism
MH  - Chemokine CCL2/antagonists & inhibitors/genetics/*metabolism
MH  - Chemotaxis/physiology
MH  - Cricetinae
MH  - Heparin/metabolism
MH  - Humans
MH  - Ligands
MH  - Mice
MH  - Protein Binding
MH  - RNA/genetics/*metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/metabolism
MH  - Surface Plasmon Resonance
EDAT- 2001/10/10 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/10 10:00
PHST- 2001/10/10 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/10 10:00 [entrez]
AID - S0014-5793(01)02895-2 [pii]
AID - 10.1016/s0014-5793(01)02895-2 [doi]
PST - ppublish
SO  - FEBS Lett. 2001 Oct 5;506(2):85-90. doi: 10.1016/s0014-5793(01)02895-2.