PMID- 11592614
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20031114
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 32
IP  - 5
DP  - 2001 Sep-Oct
TI  - Monitoring porcine reproductive and respiratory syndrome virus infection status 
      in swine herds based on analysis of antibodies in meat juice samples.
PG  - 441-53
AB  - An indirect ELISA test was developed as a novel tool aimed at monitoring the herd 
      infection status of swine herds. Meat juice samples from pig carcasses were 
      analysed for the presence of antibodies against porcine reproductive and 
      respiratory syndrome virus (PRRSV). A study of samples from herds with known PRRS 
      status was undertaken. The PRRS status of the herds was evaluated based on the 
      analysis of blood samples by another serological test (blocking ELISA) capable of 
      differentiating between infection with PRRSV of the American type and European 
      type. The specificity of the indirect ELISA test on meat juice samples was 0.98. 
      The sensitivity of the test depended on the type of the PRRSV strain involved. 
      The apparent prevalence in herds infected with the American type of PRRSV was 
      0.44. The apparent prevalence in herds infected with the European type of PRRSV 
      was 0.64. Herd level sampling and herd level criteria for assessing the PRRS 
      status of herds by the new test were developed. Herds were classified as PRRS 
      negative or PRRS seropositive based on 10 meat juice samples collected randomly 
      at slaughter throughout a 3-month-period. Herd PRRS status classification by the 
      indirect ELISA was validated in 47 herds by collection of blood samples from the 
      herds. Eighteen herds were classified as PRRS negative by both test systems. 
      Twenty-nine herds were classified as PRRS seropositive by both test systems. 
      Acceptable herd classification was achieved using this test.
FAU - Mortensen, S
AU  - Mortensen S
AD  - The National Committee for Pig Production, Danish Bacon and Meat Council, 
      Veterinary and Food Advisory Service, Copenhagen V. sm@danishmeat.dk
FAU - Strandbygaard, B
AU  - Strandbygaard B
FAU - Botner, A
AU  - Botner A
FAU - Feld, N
AU  - Feld N
FAU - Willeberg, P
AU  - Willeberg P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animal Husbandry
MH  - Animals
MH  - Antibodies, Viral/*analysis
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Meat/standards/virology
MH  - Muscle, Skeletal/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*epidemiology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Prevalence
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Time Factors
EDAT- 2001/10/11 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/10/11 10:00
PHST- 2001/10/11 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/10/11 10:00 [entrez]
AID - 10.1051/vetres:2001136 [doi]
PST - ppublish
SO  - Vet Res. 2001 Sep-Oct;32(5):441-53. doi: 10.1051/vetres:2001136.