PMID- 11593031
OWN - NLM
STAT- MEDLINE
DCOM- 20011228
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 98
IP  - 21
DP  - 2001 Oct 9
TI  - TNF-alpha -dependent maturation of local dendritic cells is critical for 
      activating the adaptive immune response to virus infection.
PG  - 12162-7
AB  - Tumor necrosis factor-alpha (TNF-alpha) is well recognized for its role in 
      mediating innate immune responses. However, the mechanisms of TNF-alpha that 
      influence the adaptive immune response to virus infections are not well 
      understood. In this study, we have investigated the role of TNF-alpha in 
      activating the cellular and humoral responses to systemic viral challenge with 
      recombinant replication-defective adenovirus (rAd). Evaluation of T cell function 
      in TNF-alpha-deficient (TNFKO) mice revealed impaired virus-specific 
      proliferation of T cells derived from the draining lymph nodes of the liver. 
      Analysis of dendritic cells (DC) isolated from local draining lymph nodes after 
      systemic challenge showed that DC from TNFKO mice were relatively immature 
      compared with those from strain-matched wild-type mice. In vitro, TNF-alpha was 
      required to mature DC efficiently during virus-mediated stimulation. Adoptive 
      transfer of primed, mature DC into TNFKO mice restored T cell responses and 
      reconstituted anti-adenovirus antibody responses. Thus, TNF-alpha plays a 
      significant role in the maturation of DC after adenovirus challenge both in vitro 
      and in vivo, highlighting the importance of this innate cytokine in activating 
      adaptive immunity to viral challenge.
FAU - Trevejo, J M
AU  - Trevejo JM
AD  - Department of Microbiology and Immunology, WJ Hearst Foundation, Weill Medical 
      College of Cornell University, New York, NY 10021, USA.
FAU - Marino, M W
AU  - Marino MW
FAU - Philpott, N
AU  - Philpott N
FAU - Josien, R
AU  - Josien R
FAU - Richards, E C
AU  - Richards EC
FAU - Elkon, K B
AU  - Elkon KB
FAU - Falck-Pedersen, E
AU  - Falck-Pedersen E
LA  - eng
GR  - P01 HL051746/HL/NHLBI NIH HHS/United States
GR  - GM07739/GM/NIGMS NIH HHS/United States
GR  - GM18268/GM/NIGMS NIH HHS/United States
GR  - F31 GM018268/GM/NIGMS NIH HHS/United States
GR  - HL51746/HL/NHLBI NIH HHS/United States
GR  - T32 GM007739/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20011002
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adaptation, Physiological/immunology
MH  - Adenoviridae Infections/*immunology
MH  - Adenoviruses, Human/immunology
MH  - Adoptive Transfer
MH  - Animals
MH  - B-Lymphocytes/immunology
MH  - Bone Marrow Cells/immunology
MH  - Cell Differentiation
MH  - Defective Viruses/immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Immunity, Active
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - T-Lymphocytes/immunology
MH  - Tumor Necrosis Factor-alpha/genetics/*immunology
PMC - PMC59785
EDAT- 2001/10/11 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/04/09
CRDT- 2001/10/11 10:00
PHST- 2001/10/11 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/11 10:00 [entrez]
PHST- 2002/04/09 00:00 [pmc-release]
AID - 211423598 [pii]
AID - 4235 [pii]
AID - 10.1073/pnas.211423598 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12162-7. doi: 10.1073/pnas.211423598. 
      Epub 2001 Oct 2.