PMID- 11593233
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20161019
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 4
IP  - 11
DP  - 2001 Nov
TI  - Visualizing synapse formation in arborizing optic axons in vivo: dynamics and 
      modulation by BDNF.
PG  - 1093-101
AB  - Dynamic developmental changes in axon arbor morphology may directly reflect the 
      formation, stabilization and elimination of synapses. We used dual-color imaging 
      to study, in the live, developing animal, the relationship between axon 
      arborization and synapse formation at the single cell level, and to examine the 
      participation of brain-derived neurotrophic factor (BDNF) in synaptogenesis. 
      Green fluorescent protein (GFP)-tagged synaptobrevin II served as a marker to 
      visualize synaptic sites in individual fluorescently labeled Xenopus optic axons. 
      Time-lapse confocal microscopy revealed that although most synapses remain 
      stable, synapses are also formed and eliminated as axons branch and increase 
      their complexity. Most new branches originated at GFP-labeled synaptic sites. 
      Increasing BDNF levels significantly increased both axon arborization and synapse 
      number, with BDNF increasing synapse number per axon terminal. The ability to 
      visualize central synapses in real time provides insights about the dynamic 
      mechanisms underlying synaptogenesis, and reveals BDNF as a modulator of 
      synaptogenesis in vivo.
FAU - Alsina, B
AU  - Alsina B
AD  - Mental Retardation Research Center, Department of Psychiatry and Biobehavioral 
      Sciences, 760 Westwood Plaza, NPI 58-258, University of California Los Angeles, 
      Los Angeles, California 90095, USA.
FAU - Vu, T
AU  - Vu T
FAU - Cohen-Cory, S
AU  - Cohen-Cory S
LA  - eng
GR  - R01 EY011912/EY/NEI NIH HHS/United States
GR  - EY11912/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (R-SNARE Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Synaptosomal-Associated Protein 25)
RN  - 0 (postsynaptic density proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Axons/*physiology/ultrastructure
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Coculture Techniques
MH  - Fluorescent Dyes/metabolism
MH  - Green Fluorescent Proteins
MH  - Image Processing, Computer-Assisted
MH  - Indicators and Reagents/metabolism
MH  - Luminescent Proteins/genetics/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - Microscopy, Confocal
MH  - Nerve Tissue Proteins/metabolism
MH  - R-SNARE Proteins
MH  - Recombinant Fusion Proteins/metabolism
MH  - Retinal Ganglion Cells/cytology/*drug effects/*physiology
MH  - Superior Colliculi/cytology/metabolism
MH  - Synapses/*physiology
MH  - Synaptosomal-Associated Protein 25
MH  - Time Factors
MH  - Xenopus laevis
EDAT- 2001/10/11 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/11 10:00
PHST- 2001/10/11 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/11 10:00 [entrez]
AID - nn735 [pii]
AID - 10.1038/nn735 [doi]
PST - ppublish
SO  - Nat Neurosci. 2001 Nov;4(11):1093-101. doi: 10.1038/nn735.