PMID- 11593531
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20061115
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 112
IP  - 4
DP  - 1999 Apr
TI  - Effects of Kupffer cells stimulated by triglyceride and very low-density 
      lipoprotein on proliferation of rat hepatic stellate cells.
PG  - 325-9
AB  - OBJECTIVE: To study the effects of triglyceride, very low-density lipoprotein 
      (VLDL), and Kupffer cell-conditioned medium (KCCM) derived from triglyceride and 
      VLDL treatment on proliferation of rat hepatic stellate cells (HSC). METHODS: HSC 
      and Kupffer cells were isolated and cultured from liver of Wistar rats by in situ 
      perfusion with proteinase and collagenase, and density gradient centrifugation 
      with Nycodenz; HSC and Kupffer cells were identified by immunohistochemistry, 
      endocytosis, and ultrastructure, etc. Kupffer cells were incubated with 
      triglyceride (25 micrograms/ml) and VLDL (25 micrograms/ml) for 24 hours, KCCM 
      were prepared, and MTT colorimetric assay was detected for HSC proliferation. 
      RESULTS: HSC proliferation was 0.1894 +/- 0.0316 (12.5 micrograms/ml), 0.1637 +/- 
      0.0243 (25 micrograms/ml), 0.1450 +/- 0.0264 (50 micrograms/ml), 0.1212 +/- 
      0.0275 (100 micrograms/ml), 0.1226 +/- 0.0138 (200 micrograms/ml) and 0.0990 +/- 
      0.0163 (400 micrograms/ml) in the presence of triglyceride and was 0.1583 +/- 
      0.0314 (6.25 micrograms/ml), 0.1642 +/- 0.0269 (12.5 micrograms/ml), 0.1834 +/- 
      0.0498 (25 micrograms/ml), 0.1964 +/- 0.0287 (50 micrograms/ml) and 0.2202 +/- 
      0.0284 (100 micrograms/ml) in presence of VLDL, respectively. Compared with the 
      control, HSC proliferation at 400 micrograms/ml of triglyceride was lower (P < 
      0.01), but at 12.5 micrograms/ml of triglyceride and 25, 50, 100 micrograms/ml of 
      VLDL higher (P < 0.05 or 0.01); HSC proliferation was 0.1569 +/- 0.0144, 0.1924 
      +/- 0.0113 and 0.1871 +/- 0.0116 in the presence of KCCM, KCCM + triglyceride and 
      KCCM + VLDL, respectively. Compared with the control and KCCM, KCCM + 
      triglyceride and KCCM + VLDL might promote HSC proliferation (P < 0.01); there 
      was no statistical significance between KCCM + triglyceride and KCCM + VLDL (P > 
      0.05); KCCM was greater in HSC proliferation than the control, but there was no 
      significant change (P > 0.05). CONCLUSIONS: Triglyceride, VLDL, and KCCM 
      stimulated by triglyceride and VLDL might promote HSC proliferation and be 
      associated with fatty liver and hepatic fibrogenesis.
FAU - Lu, L
AU  - Lu L
AD  - Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical 
      University, Shanghai 200001, China.
FAU - Zeng, M
AU  - Zeng M
FAU - Li, J
AU  - Li J
FAU - Hua, J
AU  - Hua J
FAU - Fan, J
AU  - Fan J
FAU - Fan, Z
AU  - Fan Z
FAU - Qiu, D
AU  - Qiu D
FAU - Xiao, S
AU  - Xiao S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Culture Media, Conditioned
MH  - Hepatocytes/*cytology
MH  - Kupffer Cells/*cytology
MH  - Lipid Peroxidation
MH  - Lipoproteins, VLDL/*pharmacology
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Triglycerides/*pharmacology
EDAT- 2001/10/12 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/12 10:00
PHST- 2001/10/12 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/12 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1999 Apr;112(4):325-9.